Irisin has gained attention because of its potential applications in the treatment of metabolic diseases. Accumulating evidence indicates that irisin attenuates obesity via the browning of white adipose tissue; however, the underlying mechanisms are unclear. Here, we evaluated the effects of irisin on adipocyte browning and the underlying mechanisms. The western blotting and immunofluorescence analyses demonstrated that irisin significantly induced the up-regulation of brown fat-specific proteins (PGC1α, PRDM16, and UCP-1) and HO-1 in 3T3-L1 adipocytes. Moreover, irisin significantly increased the levels of cytosolic p62 and nuclear Nrf2. These effects of irisin in the adipocytes were attenuated by treatment with SnPP or p62 siRNA. In addition, the browning effect of irisin was observed in BAT-WT-1 cells. These findings suggest that irisin induced browning effect via the p62/Nrf2/HO-1 signalling pathway and that it may be a potential candidate for preventing or treating obesity.

鸢尾素因其在代谢疾病治疗中的潜在应用而受到关注。越来越多的证据表明，虹膜素通过使白色脂肪组织变褐来减轻肥胖。但是，其潜在机制尚不清楚。在这里，我们评估了鸢尾素对脂肪细胞褐变的作用及其潜在机制。免疫印迹和免疫荧光分析表明，虹膜素显着诱导3T3-L1脂肪细胞中棕色脂肪特异性蛋白（PGC1α，PRDM16和UCP-1）和HO-1的上调。此外，鸢尾素显着增加了胞浆p62和核Nrf2的水平。通过用SnPP或p62 siRNA处理可减轻脂肪细胞中鸢尾素的这些作用。另外，在BAT-WT-1细胞中观察到了虹膜素的褐变作用。