Glycosylinositol phosphoceramide (GIPC) is a sphingophospholipid in plants. Recently, we identified that GIPC is hydrolyzed to phytoceramide 1-phosphate (PC1P) by an uncharacterized phospholipase D activity following homogenization of certain plant tissues. We now developed methods for isolation of GIPC and PC1P from plant tissues and characterized their chemical stabilities. Hydrophilic solvents, namely a lower layer of a mixed solvent system consisting of isopropanol/hexane/water (55:20:25, v/v/v) was efficient solvent for extraction and eluent in column chromatography. GIPC was isolated by Sephadex column chromatography followed by TLC. A conventional method, such as the Bligh and Dyer method, was applicable for PC1P extraction. Specifically, PC1P was isolated by TLC following mild alkali treatment of lipid extracts of plants. The yields of GIPC and PC1P in our methods were both around 50–70%. We found that PC1P is tolerant against heat (up to 125 °C), strong acid (up to 10 M HCl), and mild alkali (0.1 M KOH). In contrast, significant degradation of GIPC occurred at 100 °C and 1.0 M HCl treatment, suggesting the instability of the inositol glycan moiety in these conditions. These data will be useful for further biochemical and nutritional studies on these sphingolipids.

糖基肌醇磷酸神经酰胺（GIPC）是植物中的鞘脂磷脂。最近，我们确定GIPC在某些植物组织均质化后，通过未表征的磷脂酶D活性被水解为植物神经酰胺1-磷酸（PC1P）。现在，我们开发了从植物组织中分离GIPC和PC1P的方法，并对其化学稳定性进行了表征。亲水性溶剂，即由异丙醇/己烷/水（55:20:25，v / v / v）组成的混合溶剂系统的下层，是萃取和柱色谱中洗脱液的有效溶剂。通过Sephadex柱色谱，随后通过TLC分离GIPC。常规方法，如Bligh和Dyer方法，可用于PC1P提取。具体而言，在对植物脂质提取物进行弱碱处理后，通过TLC分离PC1P。在我们的方法中，GIPC和PC1P的产率都在50-70％左右。我们发现PC1P耐高温（最高125°C），强酸（最高10 M HCl）和弱碱（0.1 M KOH）。相反，在100°C和1.0 M HCl处理下，GIPC发生了显着降解，表明在这些条件下肌醇聚糖部分的不稳定性。这些数据将对这些鞘脂的进一步生化和营养研究有用。