We developed a new mitochondria-targeted ratiometric fluorescent probe (named PPA) for SO₂ derivatives (HSO₃^-/SO₃^2-) with a large (pseudo) Stokes shift (209 nm) based on FRET platform in which water-soluble (E)-4-(4-(dimethylamino)styryl)-1-alkylpyridin-1-ium was chosen as acceptor moiety and phenothiazine oxide as donor moiety. Probe PPA showed specific response toward HSO₃^-/SO₃^2- over H₂S, GSH, Cys and Hcy. The recognition group underwent a nucleophilic addition reaction with HSO₃^-/SO₃^2- to give rise to clear dual-emission signal changes in enhanced blue channel and subdued red channel under a single wavelength excitation. Moreover, probe PPA was successfully used for the detection of endogenous HSO₃^-/SO₃^2- and bioimaging of mitochondria-targeted in living cells.

我们开发了一种新的线粒体靶向比率荧光探针（命名PPA）为SO ₂衍生物（HSO ₃ ^- / SO ₃ ^2-）具有大的（伪）斯托克斯基于FRET平台移（209纳米），其中水溶性的（选择E）-4-（4-（二甲基氨基）苯乙烯基）-1-烷基吡啶-1-基作为受体部分，而吩噻嗪氧化物作为供体部分。探针PPA表明特定响应朝向HSO ₃ ^- / SO ₃ ^2-较H ₂ S，GSH，Cys和同型半胱氨酸。识别基发生亲核加成反应与HSO ₃ ^- / SO ₃ ^2-在单一波长激发下在增强的蓝色通道和柔和的红色通道中产生清晰的双发射信号变化。此外，探针PPA被成功用于检测内源性HSO的₃ ^- / SO ₃ ^2-和生物成像线粒体靶向在活细胞中。