In this study, a novel selective electrochemical sensor was fabricated for determination of levodopa (LD) using a glassy carbon electrode modified with MgAl-layered double hydroxide/NiMn₂O₄ functionalized polyaniline nanocomposite (LDH/NMO/PANI) and its successful formation was confirmed by various techniques. Owing to the high surface area and outstanding conductivity features provided by the combination of MgAl-LDH, NiMn₂O₄ and PANI nanoparticles, this ternary nanocomposite exhibited excellent electrochemical activity towards the detection of LD compared with the electrode modified by the pristine MgAl-LDH and MgAl-LDH/NiMn₂O₄ binary nanocomposite. In addition, enormous quantities of amine and imine functional groups on the surface of PANI lead to a strong affinity for attachment to organic molecules such as LD. The ternary nanocomposite modified electrode exhibited excellent analytical parameters such as wide linear response from 0.1 to 100 μM with very low detection limit of 0.005 μM, good reproducibility and long-term stability which are superior among the LD electrochemical sensors. Besides, the constructive sensor possessed acceptable selectivity for recognition of LD in the presence of a variety of potentially interfering species and can also effectively avoid the interference of dopamine (DA) and ascorbic acid (AA). Consequently, the LDH/NMO/PANI/GC electrode was effectively applied to detect LD in human plasma and urine samples with good recoveries, ranging from 98.0-100.1% and it shows potential usage in clinical researches.

在这项研究中，使用MgAl层状双氢氧化物/ NiMn ₂ O ₄官能化聚苯胺纳米复合材料（LDH / NMO / PANI）修饰的玻璃碳电极，制造了一种用于测定左旋多巴（LD）的新型选择性电化学传感器，并成功地形成了由各种技术证实。由于MgAl-LDH，NiMn ₂ O ₄和PANI纳米粒子的结合提供了高表面积和出色的电导特性，与原始MgAl-LDH修饰的电极相比，这种三元纳米复合材料对LD的检测显示出优异的电化学活性。和MgAl-LDH / NiMn ₂ O ₄二元纳米复合材料。另外，PANI表面上大量的胺和亚胺官能团导致与有机分子（如LD）的附着力强。三元纳米复合修饰电极表现出出色的分析参数，例如0.1至100μM的宽线性响应，0.005μM的极低检测限，良好的重现性和长期稳定性，这在LD电化学传感器中是优异的。此外，在存在多种潜在干扰物种的情况下，该结构传感器具有对LD识别的可接受的选择性，并且还可以有效避免多巴胺（DA）和抗坏血酸（AA）的干扰。因此，LDH / NMO / PANI / GC电极有效地检测了人体血浆和尿液样品中的LD，回收率良好，