The current study focused on improving the production of phenolic acids in the Woodland Sage cell suspension culture (CSC) through attaining high-yielding cell lines and carboxyl functionalized multi-walled carbon nanotubes (MWCNT-COOH) elicitation. The leaf-derived callus was irradiated at different doses of gamma irradiation 10 to 100 Gy. The maximum content of rosmarinic acid (RA), salvianolic acid B (SAB), ferulic acid (FA), and cinnamic acid (CA) was recorded in callus cultures irradiated with 70 Gy, which was 18.53, 5.21, 1.9, and 7.59 mg/g DW, respectively. The CSC that established from 70 Gy γ-irradiated calli accumulated 1.7-fold RA more higher irradiated callus culture. The CSC elicited with various concentrations of MWCNT-COOH in range 25 to 100 mg/l significantly increased fresh weight (FW), dry weight (DW), and phenolic acid contents of cells. The highest FW with 268.47 g/l and DW with 22.17 g/l was obtained in 100 mg/l MWCNT-COOH treatment. The RA, SAB, CA and FA content of CSC treated with 100 mg/l MWCNT-COOH were 13-fold, 14.2-fold, 20-fold, and 3- fold higher than wild S. nemorosa plant at flowering stage, respectively. The antioxidant activity of cultures significantly enhanced with both gamma and MWCNT-COOH based on DPPH and FRAP assay. Our results showed that the combination of cell line selection and MWCNT-COOH elicitation significantly improved the production of secondary metabolites in Woodland Sage, which is useful for large-scale production of phenolic compounds.

当前的研究集中在通过获得高产细胞系和羧基官能化的多壁碳纳米管（MWCNT-COOH）诱导来提高林地鼠尾草细胞悬浮培养（CSC）中酚酸的产生。以10至100 Gy的不同剂量的γ射线辐照叶片来源的愈伤组织。在用70 Gy照射的愈伤组织培养物中，记录了迷迭香酸（RA），丹酚酸B（SAB），阿魏酸（FA）和肉桂酸（CA）的最大含量，分别为18.53、5.21、1.9和7.59 mg / g DW。由70 Gyγ辐照的愈伤组织建立的CSC累积的辐射辐照愈伤组织培养物比RA高1.7倍。在25至100 mg / l范围内使用各种浓度的MWCNT-COOH引发的CSC显着增加了鲜重（FW），干重（DW），和细胞中的酚酸含量。在100 mg / l MWCNT-COOH处理中，最高的FW为268.47 g / l，DW为22.17 g / l。用100 mg / l MWCNT-COOH处理的CSC的RA，SAB，CA和FA含量分别比野生动物高13倍，14.2倍，20倍和3倍开花的S. nemorosa植物分别。根据DPPH和FRAP分析，γ和MWCNT-COOH均可显着提高培养物的抗氧化活性。我们的结果表明，细胞系选择和MWCNT-COOH诱导的结合显着提高了Woodland Sage中次生代谢产物的产生，可用于大规模生产酚类化合物。