The knowledge about the genetic basis of alkaline salt stress and neutral salt stress in rice is limited. Here, we located quantitative trait loci (QTL) for seed germination rate (GR) under alkaline salt stress (NaHCO 3 ) and neutral salt stress (NaCl) using backcross inbred lines population, which was derived from a cross between indica cv. Changhui 891 (CH891) and japonica cv. 02428 with 3057 bins markers. A total of ten QTL for five salt stress-related traits were detected. The QTL detected for control condition, NaCl stress, and NaHCO 3 stress were quite different, which suggested that the genes controlling the transport of the Na + , in the form of NaCl and the NaHCO 3 , may be different or induced incoordinately by salt stress. Furthermore, on the basis of the high-density genetic map, the ten QTL were mapped on 40–980 kb chromosomal regions on Nipponbare genome, and 16 candidate genes related to stress or metal transport were found. These results contribute to the fine mapping and use of these GR-related QTL.

中文翻译：

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碱性和中性盐胁迫下水稻发芽能力的高密度bin遗传图谱遗传分析

关于水稻碱性盐胁迫和中性盐胁迫的遗传基础知识有限。在这里，我们使用回交自交系群体定位了碱性盐胁迫（NaHCO 3 ）和中性盐胁迫（NaCl）下种子萌发率（GR）的数量性状基因座（QTL），该自交系来自籼稻品种之间的杂交。昌辉891（CH891）和粳稻品种。02428 带有 3057 个 bin 标记。共检测到 5 个盐胁迫相关性状的 10 个 QTL。对照条件、NaCl胁迫和NaHCO 3 胁迫检测到的QTL差异较大，这表明控制Na + 以NaCl和NaHCO 3 形式转运的基因可能不同或受盐胁迫不协调。 . 此外，在高密度遗传图谱的基础上，10个QTL定位在日本晴基因组40-980 kb染色体区域，发现16个与应激或金属转运相关的候选基因。这些结果有助于这些 GR 相关 QTL 的精细定位和使用。