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Two-stage oxygen supply strategy for enhancing fed-batch production of pyrroloquinoline quinone in Hyphomicrobium denitrificans FJNU-6.
Applied Microbiology and Biotechnology ( IF 5 ) Pub Date : 2020-06-12 , DOI: 10.1007/s00253-020-10690-0
Mengsu Liu 1 , Xinwei Yang 1 , Yang Ren 1 , Huaping Xia 1 , Jianzhong Huang 1 , Chongrong Ke 1, 2
Affiliation  

Oxygen is a vital parameter for pyrroloquinoline quinone (PQQ) biosynthesis. In this study, the effects of oxygen supply on the biosynthesis of PQQ were first investigated systematically with Hyphomicrobium denitrificans FJNU-6. Following a kinetic analysis of the specific cell growth rate (μx) and specific PQQ formation rate (μp) in 5 L benchtop fermentation systems at various oxygen supply levels ranging from 0 to 60%, a novel, two-stage oxygen supply strategy was developed for enhancing PQQ production and productivity. Moreover, the transcription of genes involved in methanol oxidation and PQQ biosynthesis was analyzed throughout the process to outline the effect of oxygen supply on cell metabolism. Furthermore, with constant feeding of methanol at 0–1 g/L after the initial methanol was consumed completely, the PQQ concentration and productivity reached 1070 mg/L and 7.64 mg/L/h, respectively, after 140 h in a 5-L fermenter. The two-stage oxygen supply strategy developed in this study provides an effective and economical strategy for the industrial production of PQQ.

Key Points

• A novel, two-stage oxygen supply strategy was developed for enhancing PQQ production and productivity.

•The transcription of genes involved in methanol oxidation and PQQ biosynthesis was regulated by changes in oxygen supply.

• This study offers an effective and economical strategy for industrial or large-scale production of PQQ.



中文翻译:

两阶段供氧策略可增强反硝化细菌FJNU-6中吡咯并喹啉醌的分批补料生产。

氧是吡咯并喹啉醌(PQQ)生物合成的重要参数。在这项研究中,首先使用反硝化微生物FJNU-6系统地研究了供氧对PQQ生物合成的影响。以下的特定的细胞生长速率的动力学分析(μ X)和比PQQ形成率(μ p)在氧气供应水平介于0%至60%的5升台式发酵系统中,开发了一种新颖的两阶段氧气供应策略,以提高PQQ的产量和生产率。此外,在整个过程中分析了参与甲醇氧化和PQQ生物合成的基因的转录,以概述氧气供应对细胞代谢的影响。此外,在初始甲醇完全消耗完后,以恒定的甲醇进料量为0-1 g / L,在5-L中140 h后,PQQ的浓度和生产率分别达到1070 mg / L和7.64 mg / L / h。发酵罐。本研究开发的两阶段供氧策略为PQQ的工业生产提供了有效而经济的策略。

关键点

•开发了一种新颖的两阶段氧气供应策略,以提高PQQ的产量和生产率。

•参与甲醇氧化和PQQ生物合成的基因的转录受供氧量变化的调节。

•该研究为工业或大规模生产PQQ提供了有效且经济的策略。

更新日期:2020-06-12
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