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An amperometric biosensor based on poly(L-aspartic acid), nanodiamond particles, carbon nanofiber, and ascorbate oxidase-modified glassy carbon electrode for the determination of L-ascorbic acid.
Analytical and Bioanalytical Chemistry ( IF 4.3 ) Pub Date : 2020-06-12 , DOI: 10.1007/s00216-020-02747-w
Ceren Kaçar 1 , Pınar Esra Erden 2
Affiliation  

An amperometric l-ascorbic acid biosensor utilizing ascorbate oxidase (AOx) immobilized onto poly(l-aspartic acid) (P(l-Asp)) film was fabricated on carbon nanofiber (CNF) and nanodiamond particle (ND)-modified glassy carbon electrode (GCE). Effects of AOx, ND, and CNF amounts were investigated by monitoring the response currents of the biosensor at different amounts of AOx, ND, and CNF. The electropolymerization step of l-aspartic acid on CNF-ND/GCE surface was also optimized. Scanning electron microscopy (SEM), cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS) techniques were used to enlighten the modification steps of the biosensor. The effects of pH and applied potential were studied in detail to achieve the best analytical performance. Under optimized experimental conditions, the AOx/P(L-Asp)/ND-CNF/GCE biosensor showed a linear response to l-ascorbic acid in the range of 2.0 × 10−7–1.8 × 10−3 M with a detection limit of 1.0 × 10−7 M and sensitivity of 105.0 μAmM−1 cm−2. The novel biosensing platform showed good reproducibility and selectivity. The strong interaction between AOx and the P(l-Asp)/ND-CNF matrix was revealed by the high repeatability (3.4%) and good operational stability. The AOx/P(l-Asp)/ND-CNF/GCE biosensor was successfully applied to the determination of l-ascorbic acid in vitamin C effervescent tablet and pharmaceutical powder containing ascorbic acid with good results, which makes it a promising approach for quantification of l-ascorbic acid.

Graphical abstract



中文翻译:

一种基于聚L-天冬氨酸,纳米金刚石颗粒,碳纳米纤维和抗坏血酸氧化酶修饰的玻璃碳电极的安培生物传感器,用于测定L-抗坏血酸。

在碳纳米纤维(CNF)和纳米金刚石颗粒(ND)修饰的玻碳电极上制备了利用抗坏血酸氧化酶(AOx)固定在聚(l-天冬氨酸)(P(l -Asp))膜上的电流型l-抗坏血酸生物传感器(GCE)。通过监测生物传感器在不同量的AOx,ND和CNF下的响应电流,研究了AOx,ND和CNF量的影响。l的电聚合步骤还优化了CNF-ND / GCE表面的天冬氨酸。扫描电子显微镜(SEM),循环伏安法(CV)和电化学阻抗谱(EIS)技术用于启发生物传感器的修饰步骤。详细研究了pH和施加电势的影响,以实现最佳分析性能。在优化的实验条件下,AOx / P(L-Asp)/ ND-CNF / GCE生物传感器对1-抗坏血酸的线性响应范围为2.0×10 -7 –1.8×10 -3  M,检测限1.0×10 -7  M的灵敏度和105.0μAmM -1  cm -2的灵敏度。新型生物传感平台显示出良好的重现性和选择性。高重复性(3.4%)和良好的操作稳定性表明了AOx与P(1 -Asp)/ ND-CNF基质之间的强相互作用。AOx / P(l -Asp)/ ND-CNF / GCE生物传感器已成功用于维生素C泡腾片和含有抗坏血酸的药物粉中的l-抗坏血酸的测定,具有良好的结果,这使其成为一种有希望的定量方法的抗坏血酸。

图形概要

更新日期:2020-06-12
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