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Preparative purification of corilagin from Phyllanthus by combining ionic liquid extraction, prep-HPLC, and precipitation.
Analytical Methods ( IF 3.1 ) Pub Date : 2020-06-11 , DOI: 10.1039/d0ay00860e
Xiaodong Hou 1 , Zitao Cheng 1 , Jiao Wang 1
Affiliation  

In this study, a green extraction and purification process for the rapid preparation of corilagin from Phyllanthus has been designed using an aqueous ionic liquid coupled with preparative high-performance liquid chromatography (prep-HPLC) and precipitation. The results showed that the optimum extraction process for corilagin involved mixing Phyllanthus tenellus Roxb. with 0.4 M [BMIm]Br at a liquid–solid ratio of 10 : 1 and dispersing the mixture by ultrasonication at 50 °C for 15 min. Macroporous resin D101 and prep-HPLC were employed for [BMIm]Br removal and corilagin separation to yield corilagin of 86.49% purity. Subsequently, corilagin was further purified by water precipitation to achieve 99.12% purity. The results indicated the successful development of a new rapid and green process to prepare corilagin on a large scale from plants using [BMIm]Br. This promising process can be applied for the preparative separation and purification of other active compounds from complex plant systems.

中文翻译:

通过将离子液体萃取,制备型HPLC和沉淀相结合,从木兰中制备可乐菌素。

在这项研究中,已设计了一种绿色提取和纯化工艺,用于从楠木中快速制备可乐菌素,方法是将水性离子液体与制备型高效液相色谱(prep-HPLC)结合并进行沉淀。结果表明,可乐菌素的最佳提取工艺包括将余甘子混合Roxb。用0.4 M [BMIm] Br以固液比10:1混合,并在50°C超声处理15分钟以分散混合物。大孔树脂D101和制备型HPLC用于去除[BMIm] Br和分离丝胶,得到纯度为86.49%的丝胶。随后,通过水沉淀进一步纯化可拉金,以达到99.12%的纯度。结果表明成功开发了一种新的快速,绿色工艺,可使用[BMIm] Br从植物中大规模制备可乐菌素。这种有前途的方法可以用于从复杂植物系统中制备分离和纯化其他活性化合物的方法。
更新日期:2020-07-09
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