Modern taxonomy requires the preservation of biospecimens for both morphological and molecular applications. The utility of a previously identified preservative, dimethyldimethylhydantoin hydantoin (Dekafald^®), to retain both physical diagnostic traits and the DNA integrity of biological specimens remains unknown. Using 439 eggs and 414 larvae from two North American fish species, we compared three hydantoin solutions at different concentrations (5%, 10%, and 20%) with gold standard preservatives (10% buffered formalin, 95% ethanol) to evaluate morphological trait retention up to 90 days, and DNA barcoding success up to 56 days. While the 5% hydantoin solution had the most sequencing success by 56 days, the 10% hydantoin solution was the best multipurpose preservative. Future work should assess the performance of ∼10% hydantoin solution over longer time periods, and its applicability to other taxa such as Arthropoda.

中文翻译：

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二甲基二甲基乙内酰脲：一种用于形态和遗传保存的替代液。

现代分类学要求保留用于形态和分子应用的生物标本。先前确定的防腐剂，dimethyldimethylhydantoin乙内酰脲的Dekafald效用（^®），如何保留物理诊断特征和生物学标本的DNA完整性仍然未知。我们使用来自北美两种鱼类的439个卵和414个幼虫，将三种浓度（5％，10％和20％）的乙内酰脲溶液与金标准防腐剂（10％缓冲福尔马林，95％乙醇）进行了比较，以评估形态特征保留长达90天，DNA条形码成功长达56天。尽管5％的乙内酰脲溶液在56天内获得最大的测序成功率，但是10％的乙内酰脲溶液是最好的多用途防腐剂。未来的工作应评估〜10％乙内酰脲溶液在较长时间内的性能，以及其在节肢动物等其他类群中的适用性。