The formation of binary self-assembled monolayers (SAMs) via the use of ammonium-terminated adsorbates offers an ionic platform for the binding of oligonucleotides and their subsequent delivery for therapeutic applications. Four different types of ammonium-terminated adsorbates were used in the present study: two ammonium-terminated and two trimethylammonium-terminated dithiols of different chain lengths, which were designed to provide a foundation for the development of a high-capacity nanoscale loading system for oligonucleotides. The maximum number of oligonucleotides immobilized on the surface was achieved by adjusting the ratio of the adsorbates in correlation with the relative packing density of the SAMs. The techniques of ellipsometry, X-ray photoelectron spectroscopy, polarization modulation infrared reflection-absorption spectroscopy, and electrochemical quartz crystal microbalance were used to analyze the SAMs. Analysis of the films revealed an optimum ratio of 75:25 of the long-chained adsorbate (ammonium-terminated) to the short-chained adsorbate (trimethylammonium-terminated) for maximum oligonucleotide loading. Further analysis indicated that burying the trimethylammonium termini into the lower interface of the monolayer provided the highest mass loading of oligonucleotides. The results presented in this study provide a foundation for the development of a gene-therapy platform when constructed on the surface of, for example, light-responsive gold nanoparticles or gold nanoshells as photo-triggerable delivery vehicles.

中文翻译：

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结合铵和三甲基铵基团的混合物终止的自组装单分子层上的DNA结合：向基因传递平台。

通过使用铵封端的吸附物形成二元自组装单分子膜（SAMs），为结合寡核苷酸及其随后的治疗应用提供了离子平台。本研究中使用了四种不同类型的铵封端的吸附物：两种不同链长的铵封端的和两种三甲基铵封端的二硫醇，旨在为开发寡核苷酸的高容量纳米级上样系统提供基础。固定在表面上的寡核苷酸的最大数量是通过调节吸附物的比例与SAM的相对堆积密度相关来实现的。椭圆偏振技术，X射线光电子能谱，偏振调制红外反射吸收光谱，用电化学石英晶体微量天平分析SAM。膜的分析表明，对于最大的寡核苷酸负载，长链被吸附物（以铵为末端）与短链被吸附物（以三甲铵为末端）的最佳比例为75:25。进一步的分析表明，将三甲基铵末端埋入单层的下部界面可提供最高的寡核苷酸质量负载。当在例如光响应性金纳米颗粒或金纳米壳作为光触发传递载体的表面上构建时，本研究中提供的结果为基因治疗平台的开发提供了基础。25个长链被吸附物（以铵为末端）至短链被吸附物（以三甲铵为末端）以最大程度地加载寡核苷酸。进一步的分析表明，将三甲基铵末端埋入单层的下部界面可提供最高的寡核苷酸质量负载。当在例如光响应性金纳米颗粒或金纳米壳作为光触发传递载体的表面上构建时，本研究中提供的结果为基因治疗平台的开发提供了基础。25个长链被吸附物（以铵为末端）至短链被吸附物（以三甲铵为末端）以最大程度地加载寡核苷酸。进一步的分析表明，将三甲基铵末端埋入单层的下部界面可提供最高的寡核苷酸质量负载。当在例如光响应性金纳米颗粒或金纳米壳作为光触发传递载体的表面上构建时，本研究中提供的结果为基因治疗平台的开发提供了基础。