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The evaluation of early stage oxidative status in streptozotocin induced diabetes in rats
Archives of Physiology and Biochemistry ( IF 3 ) Pub Date : 2020-06-10 , DOI: 10.1080/13813455.2020.1776736
Mehmet Ali Kisacam 1 , Gonca Ozan Kocamuftuoglu 2 , Hakan Ufat 3 , Sema Temizer Ozan 3
Affiliation  

Abstract

Early-stage diabetes can be defined as the stages before absolute insulin deficiency in patients. In this study, the early stage oxidative effect of streptozotocin(STZ) induced diabetes mellitus was evaluated. 28 male adult Sprague-Dawley rats were divided into four groups; control group and 7th, 14th, 21st days diabetic groups. Diabetic groups received single 65 mg/kg STZ injection intraperitoneally. Rats were decapitated at 7th, 14th and 21st days, liver tissues were taken. Nitric oxide(NO), malondialdehyde(MDA) levels and catalase, arginase activities were measured. MDA and NO levels were increased (respectively p < .001 and p < .01), mainly 14 and 21 days after STZ administration; moreover, while liver catalase activity was progressively decreased (p < .001), oppositely arginase was increased in the same time period (p < .01). Results show that MDA and nitric oxide together with catalase and arginase switch at an early stage of diabetes and they may contribute to subsequent complications related to diabetes via increased oxidative damage.



中文翻译:

链脲佐菌素诱导的大鼠糖尿病早期氧化状态的评价

摘要

早期糖尿病可以定义为患者绝对胰岛素缺乏之前的阶段。本研究评估了链脲佐菌素(STZ)诱导糖尿病的早期氧化作用。28只成年雄性Sprague-Dawley大鼠分为四组;对照组和第7、14、21天糖尿病组。糖尿病组接受单次 65 mg/kg STZ 腹腔注射。第7天、第14天和第21天将大鼠断头,取肝组织。测定一氧化氮(NO)、丙二醛(MDA)水平和过氧化氢酶、精氨酸酶活性。MDA 和 NO 水平升高(分别为p  < .001 和p  < .01),主要在 STZ 给药后 14 天和 21 天;此外,肝过氧化氢酶活性逐渐降低(p < .001),相反,精氨酸酶在同一时期增加(p  < .01)。结果表明,MDA 和一氧化氮与过氧化氢酶和精氨酸酶一起在糖尿病早期发生转变,它们可能通过增加氧化损伤而导致糖尿病相关并发症。

更新日期:2020-06-10
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