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Antibiotic-Induced Changes in Microbiome-Related Metabolites and Bile Acids in Rat Plasma.
Metabolites ( IF 4.1 ) Pub Date : 2020-06-11 , DOI: 10.3390/metabo10060242
Véronique de Bruijn 1, 2 , Christina Behr 1 , Saskia Sperber 1 , Tilmann Walk 3 , Philipp Ternes 3 , Markus Slopianka 3 , Volker Haake 3 , Karsten Beekmann 2 , Bennard van Ravenzwaay 1
Affiliation  

Various environmental factors can alter the gut microbiome’s composition and functionality, and modulate host health. In this study, the effects of oral and parenteral administration of two poorly bioavailable antibiotics (i.e., vancomycin and streptomycin) on male Wistar Crl/Wi(Han) rats for 28 days were compared to distinguish between microbiome-derived or -associated and systemic changes in the plasma metabolome. The resulting changes in the plasma metabolome were compared to the effects of a third reference compound, roxithromycin, which is readily bioavailable. A community analysis revealed that the oral administration of vancomycin and roxithromycin in particular leads to an altered microbial population. Antibiotic-induced changes depending on the administration routes were observed in plasma metabolite levels. Indole-3-acetic acid (IAA) and hippuric acid (HA) were identified as key metabolites of microbiome modulation, with HA being the most sensitive. Even though large variations in the plasma bile acid pool between and within rats were observed, the change in microbiome community was observed to alter the composition of the bile acid pool, especially by an accumulation of taurine-conjugated primary bile acids. In-depth investigation of the relationship between microbiome variability and their functionality, with emphasis on the bile acid pool, will be necessary to better assess the potential adverseness of environmentally induced microbiome changes.

中文翻译:

抗生素诱导的大鼠血浆中微生物组相关代谢产物和胆汁酸的变化。

各种环境因素可以改变肠道微生物组的组成和功能,并调节宿主健康。在这项研究中,比较了口服和肠胃外施用两种生物利用度较差的抗生素(即万古霉素和链霉素)对雄性Wistar Crl / Wi(Han)大鼠28天的影响,以区分微生物组或系统相关的变化在血浆代谢组中。将血浆代谢组中产生的变化与易于生​​物利用的第三参考化合物罗红霉素的作用进行了比较。社区分析显示,口服万古霉素和罗红霉素尤其会导致微生物种群的改变。在血浆代谢物水平上观察到了抗生素诱导的变化,这取决于给药途径。吲哚-3-乙酸(IAA)和马尿酸(HA)被确定为微生物组调节的关键代谢产物,HA是最敏感的。即使观察到大鼠之间和大鼠之间的血浆胆汁酸池有很大的变化,但观察到微生物组群落的变化会改变胆汁酸池的组成,尤其是通过牛磺酸结合的初级胆汁酸的积累。为了更好地评估环境诱导的微生物组变化的潜在不利性,有必要深入研究微生物组变异性及其功能之间的关系,重点是胆汁酸库。即使观察到大鼠之间和大鼠之间的血浆胆汁酸池有很大的变化,但观察到微生物组群落的变化会改变胆汁酸池的组成,尤其是通过牛磺酸结合的初级胆汁酸的积累。为了更好地评估环境诱导的微生物组变化的潜在不利性,有必要深入研究微生物组变异性及其功能之间的关系,重点是胆汁酸库。即使观察到大鼠之间和大鼠之间的血浆胆汁酸池有很大的变化,但观察到微生物组群落的变化会改变胆汁酸池的组成,尤其是通过牛磺酸结合的初级胆汁酸的积累。为了更好地评估环境诱导的微生物组变化的潜在不利因素,有必要深入研究微生物组变异性及其功能之间的关系,重点是胆汁酸库。
更新日期:2020-06-11
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