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Vanadium‐Substituted Tungstosulfate Polyoxometalates as Peroxidase Mimetics and Their Potential Application in Biosensing
ChemElectroChem ( IF 4 ) Pub Date : 2020-06-11 , DOI: 10.1002/celc.202000544
Ayad Saeed 1 , Muhammad Umer 2 , Naoki Yamasaki 3, 4 , Shinya Azuma 4 , Tadaharu Ueda 3, 5 , Muhammad J. A. Shiddiky 1, 2
Affiliation  

This article reports on the peroxidase‐like catalytic activity of polyoxometalates (POMs) and their potential use as natural peroxidases for developing a simple and efficient colorimetric glucose sensor. Two Keggin‐type vanadium‐substituted tungstosulfates, [SVW11O40]3− (SVW11) and [SV2W10O40]4− (SV2W10), were tested for their potential as natural enzyme mimetics and exhibited strong peroxidase‐like catalytic activity. The catalysis reaction was found to be in accordance with Michaelis‐Menten and Lineweaver‐Burk kinetics models. Michaelis‐Menten constant (Km) and maximum velocity (Vmax) parameters were calculated to be 0.0759 mM and 0.329×10−8 Ms−1 for SVW11, and 0.0543 mM and 2.67×10−8 Ms−1 for SV2W10, respectively, indicating a high catalytic activity and a strong affinity of POMs towards 3,3,5,5‐tetramethylbenzidine (TMB). In the case of H2O2, these values were found to be 57.1 mM and 0.325×10−8 mMs−1 for SVW11, and 47.7 mM and 2.72×10−8 mMs−1 for SV2W10. The peroxidase‐like catalytic activity of these POMs was used to develop colorimetric glucose sensors as a proof‐of‐concept model for the POM‐based naked‐eye detection of biomolecules. The limit of detcetion (LOD) of glucose for SVW11 and SV2W10 was 1.14 μM and 1.24 μM, respectively. Our findings propose broad‐ranging potential applications of these novel POMs in biosensing and bioanalytical chemistry.

中文翻译:

钒取代的钨硫酸盐多金属氧酸盐作为过氧化物酶模拟物及其在生物传感中的潜在应用

本文报道了多金属氧酸盐(POM)的过氧化物酶样催化活性及其作为天然过氧化物酶在开发简单有效的比色葡萄糖传感器中的潜在用途。测试了两种Keggin型钒取代的钨丝硫酸盐[SVW 11 O 40 ] 3−(SVW 11)和[SV 2 W 10 O 40 ] 4−(SV 2 W 10)作为天然酶模拟物的潜力,并展示出强大的过氧化物酶样催化活性。发现催化反应符合Michaelis-Menten和Lineweaver-Burk动力学模型。米氏常数(ķm)和最大速度(V max)参数对于SVW 11分别为0.0759 mM和0.329×10 -8 Ms -1,对于SV 2 W 10分别为0.0543 mM和2.67×10 -8 Ms -1 POM对3,3,5,5-四甲基联苯胺(TMB)具有很高的催化活性和很强的亲和力。在H 2 O 2的情况下,对于SVW 11,这些值为57.1 mM和0.325×10 -8  mMs -1,对于SV 2是47.7 mM和2.72×10 -8  mMs -110。这些POM的过氧化物酶样催化活性被用于开发比色葡萄糖传感器,作为基于POM的裸眼生物分子检测的概念验证模型。SVW 11和SV 2 W 10的葡萄糖检测极限(LOD)分别为1.14μM和1.24μM 。我们的发现提出了这些新型POM在生物传感和生物分析化学中的广泛潜在应用。
更新日期:2020-06-11
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