Phagocytosis, the ingestion of solid particles by cells is essential for nutrient uptake, innate immune response, antigen presentation and organelle homeostasis. Here we show that Lissencephaly‐1 (Lis1), a well‐known regulator of the microtubule motor dynein, co‐localizes with actin at the phagocytic cup in the early stages of phagocytosis. Both knockdown and overexpression of Lis1 perturb phagocytosis, suggesting that Lis1 levels may be regulated during particle engulfment to facilitate remodeling of actin filaments within the phagocytic cup. This requirement of Lis1 is replicated in mouse macrophage cells as well as in the amoeba Dictyostelium, indicating an evolutionarily conserved role for Lis1 in phagocytosis. In support of these findings, Dictyostelium cells overexpressing Lis1 show defects in migration possibly caused by dysregulated actin. Taken together, Lis1 localizes to the phagocytic cup and influences the actin cytoskeleton in a manner that appears important for the uptake of solid particles into cells.

中文翻译：

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Lis1 与吞噬杯中的肌动蛋白共定位并调节吞噬作用。

吞噬作用，即细胞摄取固体颗粒对于营养吸收、先天免疫反应、抗原呈递和细胞器稳态至关重要。在这里，我们展示了 Lissencephaly-1 (Lis1)，一种众所周知的微管运动动力蛋白调节剂，在吞噬作用的早期阶段与吞噬杯中的肌动蛋白共定位。Lis1 的敲低和过表达都会干扰吞噬作用，这表明 Lis1 水平可能在颗粒吞噬过程中受到调节，以促进吞噬杯内肌动蛋白丝的重塑。Lis1 的这种要求在小鼠巨噬细胞以及变形虫中复制网柄菌，表明 Lis1 在吞噬作用中的进化保守作用。为了支持这些发现，盘基网柄菌过度表达 Lis1 的细胞表现出迁移缺陷，这可能是由肌动蛋白失调引起的。总而言之，Lis1 定位于吞噬杯并以一种对将固体颗粒吸收到细胞中很重要的方式影响肌动蛋白细胞骨架。