Pluripotency is tightly regulated and is crucial for stem cells and their implementation for regenerative medicine. Non-coding RNAs, especially long non-coding RNAs (lncRNAs) emerged as orchestrators of versatile (patho)-physiological processes on the transcriptional and post-transcriptional level. Cyrano, a well-conserved lncRNA, is highly expressed in stem cells suggesting an important role in pluripotency, which we aimed to investigate in loss-off-function (LOF) experiments. Cyrano was described previously to be essential for the maintenance of mouse embryonic stem cell (ESC) pluripotency. In contrast, using different genetic models, we here found Cyrano to be dispensable in murine and human iPSCs and in human ESCs. RNA sequencing revealed only a moderate influence of Cyrano on the global transcriptome. In line, Cyrano-depleted iPSCs retained the potential to differentiate into the three germ layers. In conclusion, different methods were applied for LOF studies to rule out potential off-target effects. These approaches revealed that Cyrano does not impact pluripotency.

多能性受到严格调节，对于干细胞及其在再生医学中的实现至关重要。非编码RNA，特别是长的非编码RNA（lncRNA）在转录和转录后水平上作为多种（病理）生理过程的协调者出现。Cyrano是一种保守的lncRNA，在干细胞中高度表达，表明在多能性中起着重要作用，我们旨在研究功能丧失（LOF）实验。先前描述了Cyrano对维持小鼠胚胎干细胞（ESC）多能性至关重要。相比之下，使用不同的遗传模型，我们在这里发现了Cyrano在鼠类和人类iPSC中以及在人类ESC中都是必不可少的。RNA测序显示，西拉诺对全球转录组只有中等程度的影响。线，西拉诺耗尽的保留的iPSC的潜力分化成三种胚层。总之，将不同的方法应用于LOF研究以排除潜在的脱靶效应。这些方法表明，Cyrano不会影响多能性。