Cancer is a genomic disease associated with accumulation of genetic damage. Cancer-initiating events, such as chromosome breakage, loss and rearrangement, can be used as biomarkers to evaluate individual cancer risk. Cytokinesis-block micronucleus cytome (CBMN - Cyt) assay parameters in peripheral blood lymphocytes (PBL) of thirty four patients diagnosed with high-grade squamous intraepithelial lesions (HSIL) and fifteen healthy women were measured. The genomic instability of patients diagnosed with HSIL were investigated in order to compare differences between the two subgroups of HSIL (CIN 2 and CIN 3). The micronucleus (MN) frequencies in PBL, as well as the frequencies of nucleoplasmic bridges (NPB) and nuclear buds (NBUD) were higher in patients than in controls (Mann- Whitney test, p < 0.05). These results provide evidence that CBMN cytome assay in peripheral blood lymphocytes may be used to identify individuals who are at high risk of developing cervical cancer. Since the extent of DNA damage varies between CIN 2 and CIN 3, these findings support the CIN grading system.

癌症是一种与遗传损伤累积相关的基因组疾病。癌症起始事件，如染色体断裂、丢失和重排，可用作生物标志物来评估个体癌症风险。测量了诊断为高度鳞状上皮内病变 (HSIL) 的 34 名患者和 15 名健康女性的外周血淋巴细胞 (PBL) 中的细胞分裂阻滞微核细胞组 (CBMN-Cyt) 测定参数。为了比较 HSIL 的两个亚组（CIN 2 和 CIN 3）之间的差异，研究了诊断为 HSIL 的患者的基因组不稳定性。PBL 中的微核 (MN) 频率以及核质桥 (NPB) 和核芽 (NBUD) 的频率在患者中高于对照组（Mann-Whitney 检验，p < 0.05）。这些结果提供了证据，表明外周血淋巴细胞中的 CBMN 细胞组测定可用于识别患宫颈癌的高风险个体。由于 DNA 损伤的程度在 CIN 2 和 CIN 3 之间有所不同，这些发现支持 CIN 分级系统。