The photoinduced charge separation in Q_B-depleted reaction centers (RCs) from Rhodobacter sphaeroides R-26 in solid air-dried and vacuum-dried (~10⁻² Torr) films, obtained in the presence of detergent n-dodecyl-β-D-maltoside (DM), is characterized using ultrafast transient absorption spectroscopy. It is shown that drying of RC-DM complexes is accompanied by reversible blue shifts of the ground-state absorption bands of the pigment ensemble, which suggest that no dehydration-induced structural destruction of RCs occurs in both types of films. In air-dried films, electron transfer from the excited primary electron donor P^⁎ to the photoactive bacteriopheophytin H_A proceeds in 4.7 ps to form the P⁺H_A⁻ state with essentially 100% yield. P⁺H_A⁻ decays in 260 ps both by electron transfer to the primary quinone Q_A to give the state P⁺Q_A⁻ (87% yield) and by charge recombination to the ground state (13% yield). In vacuum-dried films, P^⁎ decay is characterized by two kinetic components with time constants of 4.1 and 46 ps in a proportion of ~55%/45%, and P⁺H_A⁻ decays about 2-fold slower (462 ps) than in air-dried films. Deactivation of both P^⁎ and P⁺H_A⁻ to the ground state effectively competes with the corresponding forward electron-transfer reactions in vacuum-dried RCs, reducing the yield of P⁺Q_A⁻ to 68%. The results are compared with the data obtained for fully hydrated RCs in solution and are discussed in terms of the presence in the RC complexes of different water molecules, the removal/displacement of which affects spectral properties of pigment cofactors and rates and yields of the electron-transfer reactions.

Q中的光诱导电荷分离_乙从耗尽的反应中心（RCS）球形红细菌R-26在固体空气干燥，真空干燥（约10 ^-2 乇）膜，在洗涤剂的存在下获得Ñ -dodecyl- β - D-麦芽糖苷（DM）使用超快速瞬态吸收光谱法进行表征。结果表明，RC-DM配合物的干燥伴随着颜料系基态吸收带的可逆蓝移，这表明在两种类型的薄膜中均未发生脱水引起的RCs结构破坏。在风干膜中，电子从受激的一次电子供体^P⁎转移至光敏细菌^脱镁叶绿素H _A在4.7 PS进行到形成P ⁺ ħ_甲^-用基本上100％的产率状态。P ⁺ ħ_甲^-在两个通过电子转移到主醌Q 260皮秒衰变_甲，得到状态P ⁺ Q_甲^-（87％产率），并通过电荷复合到基态（13％收率）。在真空干燥后的膜，P ^⁎衰变的特征在于两个动能部件与4.1时间常数和46个PS中的〜55％/ 45％的比例，和P ⁺ ħ_甲^-衰变约2倍的速度较慢（462 ps）的比风干胶片 停用^P⁎和P ⁺ħ_甲^-基态有效地与在真空干燥的RC的相应的正向电子转移反应，降低P的产量竞争⁺ Q_甲^- 68％。将结果与在溶液中完全水合的RCs所获得的数据进行比较，并就RC络合物中不同水分子的存在进行了讨论，水分子的去除/置换会影响颜料辅因子的光谱性质以及电子的速率和产率-转移反应。