In peach, pollen sterility, expressed as absence of pollen in the anthers, segregates as an undesired trait in breeding programs. Pollen fertility screening in progenies is not a common practice mainly because it does not affect fruit set since cross-pollination is frequent. It is also a time-consuming activity that coincides with the busy pollination season. Segregation for this trait could be avoided by using molecular markers to identify appropriate parents or male sterile plants for early culling in progenies expected to segregate, thus increasing breeding efficiency. In peach, pollen sterility is determined by a recessive allele in homozygosis of the major gene, Ps/ps, located on chromosome 6. In this work, using a conventional mapping approach combined with bulked segregant analysis using resequencing data, we fine mapped Ps to a region of almost 160 kb and developed molecular markers for marker-assisted breeding. These markers were validated in plant materials from three peach breeding programs, including progenies, advanced selections, and cultivars, allowing us to determine that the frequency of the ps allele is high (0.23) and also to infer the genotypes of a large collection of cultivars and advanced breeding lines.

在桃子中，花粉不育（表示为花药中没有花粉）在育种程序中作为不希望的性状分离。在子代中进行花粉育性筛查并不是一种常见的做法，主要是因为它不影响坐果，因为异花授粉很常见。这也是一项耗时的活动，正值繁忙的授粉季节。通过使用分子标记物鉴定合适的亲本或雄性不育植物，以便在预期分离的后代中进行早期淘汰，可以避免该性状的分离。在桃子中，花粉不育由主要基因Ps / ps纯合的隐性等位基因决定，位于第6号染色体上。在这项工作中，我们使用常规的作图方法结合使用重测序数据的大量分离物分析，将Ps精细化到了约160 kb的区域，并开发了用于标记辅助育种的分子标记。这些标记已在三个桃子育种计划的植物材料中得到验证，包括后代，高级选择和品种，从而使我们能够确定ps等位基因的频率很高（0.23），并可以推断出大量品种的基因型。和先进的育种系。