We evaluated the effects of Rubus tereticaulis in healing process by determining the total carbonyl content, collagen synthesis, and total protein level on rat wounded tissues. Wounds were performed in the back of 54 Wistar rats, using a biopsy punch instrument with 0.6 mm in diameter. Rats were randomly divided into three groups: (i) un-treatment wounds group served as “controls”, (ii) Madecassol® used as “positive control” group, and (iii) the application of topical cream of R. tereticaulis served as “treatment” group of wound healing. The animals were killed at the end of experiment under anesthesia with ketamine, and tissue samples were collected for the evaluation at three times intervals (3rd, 7th, and 14th day). The wounded areas were analyzed for total carbonyl content, collagen, and total protein levels by HPLC, ELISA, and spectrophotometric methods, respectively. Total carbonyl content in the treatment group was significantly lower in comparison with control group on 3rd day (2.839 ± 0.438 vs. 3.216 ± 0.216 nmol carbonyl/mol protein; p < 0.5) and 14th days (4.222 ± 0.128 vs. 4.784 ± 0.077 nmol carbonyl/mol protein; p < 0.05), respectively. New collagen formation on the wound sites after the initial injury was noted in the treated and positive control groups (5.310 ± 0.331 vs. 5.164 ± 0.377 mg collagen/g wet tissue) at the 3rd day than control group (2.180 ± 0.718 mg collagen/g wet tissue, p < 0.01), and in treated and positive control groups at 7th day (9.654 ± 0.201, 9.053 ± 1.062 mg collagen/g wet tissue, p < 0.01); and in treated and positive control groups at 14th day (8.469 ± 0.236, 5.631 ± 0.531 mg collagen/g wet tissue, respectively; p < 0.05) in comparison with the control group. Total protein level of samples did not change significantly between the groups. Thus, application of R. tereticaulis ameliorated the wound healing process in rats as it facilitated collagen formation through healing of the wound. Evaluating total carbonyl content by HPLC could be useful as an advance procedure for quantification of healing.

我们通过确定大鼠受伤组织上的总羰基含量，胶原蛋白合成和总蛋白水平，评估了悬钩子在愈合过程中的作用。使用直径为0.6 mm的活检穿孔器在54只Wistar大鼠的背部进行伤口。将大鼠随机分为三组：（i）未经治疗的伤口组作为“对照组”，（ii）Madecassol®用作“阳性对照组”，以及（iii）涂R. tereticaulis的局部乳膏曾作为伤口愈合的“治疗”组。在实验结束时，在氯胺酮麻醉下处死动物，并以三个时间间隔（第3、7和14天）收集组织样品进行评估。分别通过HPLC，ELISA和分光光度法分析了受伤部位的总羰基含量，胶原蛋白和总蛋白水平。与对照组相比，治疗组在第3天（2.839±0.438 vs. 3.216±0.216 nmol羰基/ mol蛋白；p  <0.5）和第14天（4.222±0.128 vs. 4.784±0.077 nmol）明显低于对照组。羰基/摩尔蛋白; p <0.05）。在治疗第3天，治疗组和阳性对照组（5.310±0.331 vs. 5.164±0.377 mg胶原/ g湿组织）中发现了初始损伤后伤口部位新的胶原蛋白形成，而对照组（2.180±0.718 mg胶原/ g湿组织，p  <0.01），以及治疗组和阳性对照组在第7天时（9.654±0.201，9.053±1.062 mg胶原蛋白/ g湿组织，p  <0.01）；与治疗组和阳性对照组相比，第14天（分别为8.469±0.236、5.631±0.531 mg胶原/ g湿组织；p  <0.05）。两组之间样品的总蛋白水平没有显着变化。因此，R。tereticaulis的应用改善了大鼠的伤口愈合过程，因为它通过伤口愈合促进了胶原蛋白的形成。通过HPLC评估总羰基含量可以用作定量治愈的先进方法。