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Identification of Functional SSR Markers in Freshwater Ornamental Shrimps Neocaridina denticulata Using Transcriptome Sequencing.
Marine Biotechnology ( IF 3 ) Pub Date : 2020-06-11 , DOI: 10.1007/s10126-020-09979-y
Chang-Wen Huang , Pei-Yun Chu , Yu-Fang Wu , Wei-Ren Chan , Yeh-Hao Wang

The amazing colors and patterns are fascinating characteristics in all of the aquarium species. However, genetic and breeding molecular investigations of ornamental shrimps are rather limited. Here, we present the first transcriptomic analysis and application of microsatellites based on the chromatophore-encoded genes of Neocaridina denticulata to assist freshwater ornamental shrimp germplasm enhancement and its extensive applications. A total of 65,402 unigenes were annotated, and 4706 differentially expressed genes were screened and identified between super red shrimp and chocolate shrimp strains. Several gene ratios were examined to put in perspective possible genetic markers for the different strains of normal pigmentation development, including flotillin-2-like, keratin, the G protein–coupled receptor Mth2-like, annexin A7, and unconventional myosin-IXb-like. Five simple sequence repeat markers were effective for colored shrimps and were used to develop a marker-assisted selection platform for systematic breeding management program to maintain genetic diversity of the species. These markers could also be used to assist the identification of pure strains and increase the genetic stability of ornamental shrimp color phenotypes. Consequently, our results of microsatellite marker development are valuable for assisting shrimp genetic and selection breeding studies on freshwater ornamental shrimp and related crystal shrimp species.



中文翻译:

使用转录组测序鉴定淡水观赏虾新鳞螨中的功能性SSR标记。

惊人的颜色和图案是所有水族馆物种的迷人特征。然而,观赏虾的遗传和繁殖分子研究相当有限。在这里,我们介绍新的基于微型树新染色体的染色体编码基因的微卫星转录组学分析和应用协助淡水观赏虾种质增强及其广泛应用。总共对65,402个单基因进行了注释,并筛选和鉴定了4706个差异表达基因,它们在超级红虾和巧克力虾菌株之间鉴定。研究了几种基因比率,以阐明正常色素沉着发展的不同菌株的可能遗传标记,包括弗洛林-2样,角蛋白,G蛋白偶联受体Mth2样,膜联蛋白A7和非常规的肌球蛋白IXb样。 。五个简单的序列重复标记对有色虾有效,并被用于开发标记辅助选择平台,以进行系统的育种管理程序,以维持该物种的遗传多样性。这些标记还可以用于协助鉴定纯菌株,并提高观赏虾颜色表型的遗传稳定性。因此,我们的微卫星标记开发结果对于协助虾类遗传育种和淡水观赏虾及相关水晶虾种类的选择育种具有重要价值。

更新日期:2020-06-11
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