Diffuse large B-cell lymphoma (DLBCL) is the most common subtype of malignant non-Hodgkin lymphoma cases. An increasing body of evidence has indicated the critical roles of microRNAs (miRNAs) in regulating the progression of DLBCL. In this study, we found that miR-645 was up-regulated in DLBCL tissues and cell lines. Down-regulation of miR-645 significantly inhibited the proliferation, cell cycle progression and promoted the apoptosis of DLBCL cells. Experimental study identified Dachshund family transcription factor 1 (DACH1) as a target of miR-645. MiR-645 bound the 3′-untranslated region of DACH1 and reduced the expression of DACH1 in DLBCL cells. Decreased expression of DACH1 was inversely correlated with that of miR-645 in DLBCL tissues. The promoting effect of miR-645 on the proliferation of DLBCL cells was attenuated with the overexpression of DACH1. These results demonstrated the novel mechanism of miR-645 in DLBCL, which indicated miR-645 as a potential target for the diagnosis and prognostics of DLBCL.

弥漫性大B细胞淋巴瘤（DLBCL）是恶性非霍奇金淋巴瘤病例中最常见的亚型。越来越多的证据表明，microRNA（miRNA）在调节DLBCL进程中起着至关重要的作用。在这项研究中，我们发现miR-645在DLBCL组织和细胞系中上调。miR-645的下调显着抑制了DLBCL细胞的增殖，细胞周期进程并促进了其凋亡。实验研究确定达克斯猎​​犬家族转录因子1（DACH1）为miR-645的靶标。MiR-645结合DACH1的3'-非翻译区，并降低DLBCL细胞中DACH1的表达。在DLBCL组织中，DACH1的表达降低与miR-645的表达呈负相关。随着DACH1的过度表达，miR-645对DLBCL细胞增殖的促进作用减弱。这些结果证明了miR-645在DLBCL中的新颖机制，表明miR-645是DLBCL的诊断和预后的潜在靶标。