Careya arborea (CA) is a plant found in Thailand and consumed daily as a fresh vegetable. This study evaluated the effects of treatment with CA against human breast cancer, MCF-7 cell lines. Effects of ethanolic extract of CA leaves were measured on cell viability, migration and its mechanism. Methods were performed by sulforhodamine B (SRB), colony formation, wound healing, gelatin zymography, RT-PCR and Western blotting assay. The result indicated that CA inhibited MCF-7 cancer cell viability in a concentration-dependent manner with very low IC₅₀ values of 34.78 ± 1.30 and 18.88 ± 2.18 μg/mL after treatment with 24 and 48 h, respectively. CA inhibited colony formation with a lower IC₅₀ value of 3.48 ± 0.15 μg/mL than found from cell death. The studies showed that CA leaf extracts enhanced the process of apoptosis by inducing significant ROS generation, increasing caspase 3 activity and consistently reducing mitochondrial function. Additionally, CA leaf extracts significantly reduced cyclin D1 protein along with increasing caspase 3 and cytochrome c protein. Interestingly, low concentrations CA leaf extract (5 μg/mL) induced greater apoptotic bodies and cell shrinking compared to the untreated control groups. Finally, CA leaf extract potently suppressed the migration of MCF-7 cells accompanied by downregulation of the MMP 2 and MMP 9 protein expression. These results indicate that CA leaf extract has an anticancer activity against MCF-7 cells and is maybe useful for breast cancer prevention and therapy.

Careya arborea（CA）是在泰国发现的植物，每天作为新鲜蔬菜食用。这项研究评估了CA对人乳腺癌MCF-7细胞系的治疗效果。测定了CA叶乙醇提取物对细胞活力，迁移及其机制的影响。方法通过磺基罗丹明B（SRB），菌落形成，伤口愈合，明胶酶谱，RT-PCR和Western印迹法进行。结果表明，CA分别以24和48 h处理后以浓度依赖性的方式抑制MCF-7癌细胞的活力，其IC ₅₀值非常低，分别为34.78±1.30和18.88±2.18μg/ mL。CA以较低的IC ₅₀抑制菌落形成比细胞死亡发现的值高3.48±0.15μg/ mL。研究表明，CA叶提取物通过诱导显着的ROS产生，增加caspase 3活性并持续降低线粒体功能来增强细胞凋亡过程。此外，CA叶提取物显着减少了细胞周期蛋白D1蛋白，同时增加了胱天蛋白酶3和细胞色素c蛋白。有趣的是，与未处理的对照组相比，低浓度的CA叶提取物（5μg/ mL）诱导了更大的凋亡小体和细胞萎缩。最后，CA叶提取物有效抑制了MCF-7细胞的迁移，并伴随MMP 2和MMP 9蛋白表达的下调。这些结果表明CA叶提取物具有针对MCF-7细胞的抗癌活性，并且可能对乳腺癌的预防和治疗有用。