当前位置: X-MOL 学术Arch. Virol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Infectious bronchitis virus inhibits activation of the TLR7 pathway, but not the TLR3 pathway.
Archives of Virology ( IF 2.7 ) Pub Date : 2020-06-10 , DOI: 10.1007/s00705-020-04690-8
Jinyan Zhu 1 , Shuang Xu 1 , Xueyan Li 1 , Jue Wang 1 , Yueqi Jiang 1 , Weichen Hu 1 , Wenke Ruan 1
Affiliation  

Various strains of infectious bronchitis virus (IBV) cause different forms of infectious bronchitis with different clinical signs. Here, primary chicken embryo kidney (CEK) cells and specific-pathogen-free (SPF) chickens were infected with three pathogenic IBV strains, and it was observed that the TLR7-MYD88 pathway was inhibited but the TLR3-TIRF pathway was activated. After treatment with poly(I:C)-LMW, poly (I:C)-LMW/LyoVec, and Imiquimod, the replication of IBV was significantly suppressed after 24 h. However, treatment with TLR3 pathway inhibitors such as Pepinh-TRIF, celastrol, chloroquine, and BX795 resulted in increased replication of IBV after 36 h. These results also showed that chloroquine and celastrol were most effective inhibitors of the antiviral response at 48 hpi.



中文翻译:

传染性支气管炎病毒抑制TLR7途径的激活,但不抑制TLR3途径的激活。

各种类型的传染性支气管炎病毒(IBV)导致具有不同临床体征的不同形式的传染性支气管炎。在此,用三种致病性IBV株感染了原代鸡胚肾(CEK)细胞和无特定病原体(SPF)鸡,并且观察到TLR7-MYD88途径受到抑制,但TLR3-TIRF途径被激活。用聚(I:C)-LMW,聚(I:C)-LMW / LyoVec和咪喹莫特治疗后,IBV的复制在24小时后被显着抑制。但是,使用TLR3途径抑制剂(例如Pepinh-TRIF,西来那妥,氯喹和BX795)进行治疗会导致36小时后IBV复制增加。这些结果还表明,氯喹和Celastrol是48 hpi时最有效的抗病毒反应抑制剂。

更新日期:2020-06-10
down
wechat
bug