Scorpion venom contains various biomolecules with potential therapeutic values against different diseases, including cancer. The present study was carried out to assess the antitumor efficacy of Androctonus australis crude venom using both in vitro and in vivo approaches. For in vitro assay, the cytotoxic effect of different venom concentrations was determined against HCT116, HepG2, MCF-7, and PC-3 as cancer cell lines and normal WISH cell line. The in vivo assay was carried out by the I.P. transplantation of EAC into Swiss albino female mice, followed by the I.P. injection of the venom at the sublethal dose 1/10 LD50 (0.025 mg/kg BW) compared to cisplatin (2 mg/kg BW), and both normal and EAC control groups were also included. The analysis of ascetic fluid tumor, survival study, and hematological, biochemical, antioxidant, and histopathological assays was evaluated in control and treated animal groups. Our in vitro results revealed that the A. australis venom had a selective promising activity against MCF-7 cells (IC50 = 19.71 μg/mL). Moreover, it was less cytotoxic on WISH cells. The in vivo data showed that A. australis venom exhibited a highly significant decrease in tumor volume, and viable tumor cell count, and increased the duration of lifespan compared to the EAC control group. The venom significantly enhanced both hematological and biochemical measurements compared to the EAC control group. The results revealed that the A. australis venom exhibited in vitro and in vivo antitumor activities. Further venomics studies are needed to functionally characterize the active molecules from this scorpion venom and study their mode of action on cancer cells to develop them into potential anticancer agents.

中文翻译：

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埃及蝎毒 Androctonus Australis 的抗肿瘤功效：体外和体内研究

蝎毒含有多种生物分子，对包括癌症在内的不同疾病具有潜在治疗价值。本研究旨在使用体外和体内方法评估 Androctonus australis 粗毒液的抗肿瘤功效。对于体外试验，测定了不同毒液浓度对 HCT116、HepG2、MCF-7 和 PC-3 作为癌细胞系和正常 WISH 细胞系的细胞毒性作用。体内试验是通过将 EAC 腹腔内移植到瑞士白化雌性小鼠体内，然后腹腔注射亚致死剂量 1/10 LD50 (0.025 mg/kg BW) 与顺铂 (2 mg/kg BW) 相比的毒液BW)，并且还包括正常和 EAC 对照组。腹水肿瘤分析、生存研究、血液学、生化、抗氧化、在对照组和治疗组中评估组织病理学测定。我们的体外结果显示 A. australis 毒液对 MCF-7 细胞具有选择性的有前景的活性 (IC50 = 19.71 μg/mL)。此外，它对 WISH 细胞的细胞毒性较小。体内数据显示，与 EAC 对照组相比，澳大利亚曲霉菌毒液的肿瘤体积和存活肿瘤细胞计数显着降低，并延长了寿命。与 EAC 对照组相比，毒液显着增强了血液学和生化测量。结果表明，A. australis 毒液表现出体外和体内抗肿瘤活性。