We developed the microfluidic co-culture platform to study photothermal therapy applications. We conjugated folic acid (FA) to target breast cancer cells using reduced graphene oxide (rGO)-based functional nanomaterials. To characterize the structure of rGO-based nanomaterials, we analyzed the molecular spectrum using UV–visible and Fourier-transform infrared spectroscopy (FT-IR). We demonstrated the effect of rGO-FA-based nanomaterials on photothermal therapy of breast cancer cells in the microfluidic co-culture platform. From the microfluidic co-culture platform with breast cancer cells and human umbilical vein endothelial cells (HUVECs), we observed that the viability of breast cancer cells treated with rGO-FA-based functional nanomaterials was significantly decreased after near-infrared (NIR) laser irradiation. Therefore, this microfluidic co-culture platform could be a potentially powerful tool for studying cancer cell targeting and photothermal therapy.

中文翻译：

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rGO纳米材料介导的癌症靶向和微流体共培养平台中的光热疗法

我们开发了微流体共培养平台来研究光热疗法的应用。我们使用减少的氧化石墨烯（rGO）为基础的功能纳米材料将叶酸（FA）结合到靶向乳腺癌细胞上。为了表征基于rGO的纳米材料的结构，我们使用紫外可见光和傅立叶变换红外光谱（FT-IR）分析了分子光谱。我们展示了基于rGO-FA的纳米材料在微流体共培养平台中对乳腺癌细胞的光热治疗的效果。从具有乳腺癌细胞和人脐静脉内皮细胞（HUVEC）的微流体共培养平台，我们观察到基于rGO-FA的功能纳米材料处理的乳腺癌细胞的活力在近红外（NIR）激光照射后显着降低辐射。因此，