Securidaca longipedunculata Fresen is an indigenous medicinal plant in Africa that has an important place in both traditional and modern medicine. This plant is endangered because of high seed dormancy, low germination rate, and over exploitation. Therefore, micropropagation method is important to address these problems. The objective of this study is to develop a micropropagation protocol for S. longipedunculata from shoot tip explants. Among different Clorox concentrations, seeds sterilized with 10% Clorox for 10 min resulted in 85% decontamination and 80% germination. Among different media used to evaluate the rate of seed germination, seeds that were de-coated and transversally cut at the tip and cultured on basal MS medium resulted in 100% germination. The highest percentage of shoot initiation (87%) was obtained on MS medium containing 1.0 mg/l 6-Benzylaminopurine (BAP). The highest mean shoot number per explant (8.5 ± 0.69) was achieved on MS multiplication medium containing 1.5 mg/l BAP in combination with 0.1 mg/l Indole-3-butyric acid (IBA). The highest mean number of roots per explant (3.73 ± 0.69) was obtained on MS medium containing 2.0 mg/l Indole-3-acetic-acid (IAA). Among plantlets transferred to greenhouse, 60% survived after acclimatization. This micropropagation protocol can be used for mass propagation of S. longipedunculata that contributes to its conservation and genetic improvement.

中文翻译：

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尖梢Securidaca longipedunculata（Fresen）的体外繁殖：一种濒危药用植物

Securidaca longipedunculata Fresen是非洲的本土药用植物，在传统医学和现代医学中均占有重要地位。该植物由于种子休眠高，发芽率低和过度开发而受到威胁。因此，微传播方法对于解决这些问题很重要。这项研究的目的是开发从茎尖外植体中长链沙门氏菌的微繁殖方案。在不同的Clorox浓度中，用10％Clorox灭菌10分钟的种子导致85％的去污和80％的发芽。在用于评估种子发芽率的不同培养基中，去涂层并在尖端切成横向并在基础MS培养基上培养的种子可100％萌发。在包含1的MS培养基上获得最高的芽萌生百分比（87％）。0毫克/升6-苄氨基嘌呤（BAP）。在含有1.5 mg / l BAP和0.1 mg / l吲哚-3-丁酸（IBA）的MS繁殖培养基上，获得最高的平均外植芽数（8.5±0.69）。在含有2.0 mg / l吲哚-3-乙酸（IAA）的MS培养基上获得最高的平均外植体根数（3.73±0.69）。在适应温室的小苗中，有60％存活下来。该微繁殖方案可用于长链沙门氏菌的大量繁殖，这有助于其保存和遗传改良。在含有2.0 mg / l吲哚-3-乙酸（IAA）的MS介质上获得69）。在适应温室的小苗中，有60％存活下来。该微繁殖方案可用于长链沙门氏菌的大量繁殖，这有助于其保存和遗传改良。在含有2.0 mg / l吲哚-3-乙酸（IAA）的MS介质上获得69）。在适应温室的小苗中，有60％存活下来。该微繁殖方案可用于长链沙门氏菌的大量繁殖，这有助于其保存和遗传改良。