In the present study, previously isolated, safe, and avirulent enterococci strains were exploited for their metabolic profile (Bhagwat et al., Asian J Pharm Clin Res 12: 2019). Thirteen enterococci strains of human origin produced important enzymes like amylase (0.5–0.7 mg ml−1), protease (192–264 mg ml−1), lipase (8–10 mg ml−1), bile salt hydrolase, conjugated linoleic acid (CLA), and lactic acid (highest 12 mg ml−1), thus implicating potential attributes of starter cultures in food and dairy industry. Biogenic amines like arginine and tryptamine were produced after 4 days above 25 °C. Castor oil (highest yield 60 μg ml−1) and sunflower oil (highest yield 48 μg ml−1) both proved to be excellent sources of CLA production. Reduction assays using FRAP, ABTS (above 83%), and DPPH (30–50%) revealed excellent radical scavenging properties of cell-free supernatants of Enterococcus strains. The results implicate the future potential of application enterococci for therapeutic purpose as well as the food industry.

中文翻译：

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人源肠球菌菌株代谢谱的体外评估

在本研究中，利用先前分离的，安全的和无毒的肠球菌菌株进行新陈代谢（Bhagwat等人，Asian J Pharm Clin Res 12：2019）。13种人源肠球菌菌株产生了重要的酶，例如淀粉酶（0.5–0.7 mg ml-1），蛋白酶（192–264 mg ml-1），脂肪酶（8–10 mg ml-1），胆盐水解酶，共轭亚油酸（CLA）和乳酸（最高12 mg ml-1），因此暗示了食品和乳制品行业中发酵剂培养物的潜在特性。在25°C以上4天后产生了诸如精氨酸和色胺等生物胺。蓖麻油（最高产量60μgml-1）和葵花籽油（最高产量48μgml-1）均被证明是CLA生产的极佳来源。使用FRAP，ABTS的还原分析（超过83％），和DPPH（30-50％）表现出优异的清除肠球菌菌株无细胞上清液的自由基清除性能。结果暗示了将肠球菌用于治疗目的以及食品工业的未来潜力。