Salmonella is considered to be the second largest source of infection in food-borne diseases. It is also considered one of the most important dangers particularly in the meat and dairy industry. Therefore, the main objective of our study was to determine the relationship between thermotolerance of a Salmonella serotype and the expression of DnaK and HtrA genes. In this study, expression of the two genes DnaK and HtrA was compared under four different temperatures 37 °C, 42 °C, 50 °C, and 55 °C in two serotypes of Salmonella enterica subsp. enterica. One of them was isolated from tahini product and identified as Salmonella enterica subsp. enterica serovar choleraesuis. This identified serotype was found to be more thermotolerant than the second serotype (Salmonella enterica subsp. enterica serovar typhimurium (ATCC 13311)), which was used as reference. This conclusion was based on D and Z values, which were used to compare thermoresistance ability of the two serotypes under four different temperatures 60 °C, 65 °C, 70 °C, and 75 °C. In addition, the results of qRT-PCR showed that after 43 °C (induction temperature), the relative expression (fold change) of DnaK and HtrA genes increased up to 5 and 47, respectively, comparing to their expression at 37 °C. Thermotolerance of the identified S. choleraesuis serotype showed significantly high expression levels of DnaK and HtrA genes.

中文翻译：

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的表达的DnaK和的hTRA在高温下的基因及其上的的耐热冲击 沙门氏菌从芝麻酱产物中分离血清型

沙门氏菌被认为是食源性疾病的第二大传染源。它也被认为是最重要的危险之一，尤其是在肉类和奶业中。因此，我们研究的主要目的是确定沙门氏菌血清型的耐热性与DnaK和HtrA基因表达之间的关系。在这项研究中，比较了两种血清型沙门氏菌亚种在37°C，42°C，50°C和55°C四种不同温度下两个基因DnaK和HtrA的表达。肠 其中之一是从芝麻酱产品中分离出来的，被鉴定为肠沙门氏菌亚种。猪霍乱肠炎。发现该鉴定出的血清型比第二种血清型（沙门氏菌肠亚种肠型鼠伤寒沙门氏菌（ATCC 13311））更具耐热性，用作参考。该结论基于D和Z值，用于比较两种血清型在四种不同温度（60°C，65°C，70°C和75°C）下的耐热性。此外，qRT-PCR的结果显示，在43°C（诱导温度）后，与在37°C的表达相比，DnaK和HtrA基因的相对表达（倍数变化）分别增加至5和47。鉴定的霍乱链球菌血清型的耐热性显示DnaK和HtrA基因的表达水平显着高。与在37°C的表达相比，DnaK和HtrA基因的相对表达（倍数变化）分别增加了5和47。鉴定的霍乱链球菌血清型的耐热性显示DnaK和HtrA基因的表达水平显着高。与在37°C的表达相比，DnaK和HtrA基因的相对表达（倍数变化）分别增加了5和47。鉴定的霍乱链球菌血清型的耐热性显示DnaK和HtrA基因的表达水平显着高。