To support the multicomponent pharmacokinetics of Gastrodia elata, a rapid, simple, and sensitive ultra-performance liquid chromatography tandem with mass spectrometry (LC–MS/MS) approach was established for simultaneous quantification of gastrodin, parishin A, parishin B, parishin C, and parishin E. Five compounds were extracted from plasma by using one-step protein precipitation. The chromatographic separation was achieved on a C18 column with gradient mobile phase comprising acetonitrile and 0.05% formic acid. The detection was performed using negative electrospray ionization in multiple reaction monitoring mode. This new method maximizes assay throughput by using minimal sample clean-up procedures and a shorter analytical run time. The approach exhibited good linearity for the five compounds (r2 > 0.995) in the concentration ranges. The lower limits of quantification (LLOQ) were determined as 1.37 ng/mL for parishin A, parishin B, parishin C, and parishin E and 10 ng/mL for gastrodin. Then the method was fully validated with intra- and inter-day precision, accuracy, matrix effects, extraction recovery, and stability. This validated approach was successfully applied to the pharmacokinetic study following oral administration of Gastrodia elata extract to rats. This investigation may provide some guidance for the clinical application and explanation of the pharmacological mechanism of Gastrodia elata. Graphical abstract Graphical abstract

中文翻译：

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一种简化的 LC-MS/MS 方法，用于在大鼠口服天麻提取物后同时定量和药代动力学中的五种化合物

为了支持天麻的多组分药代动力学，建立了一种快速、简单、灵敏的超高效液相色谱串联质谱 (LC-MS/MS) 方法，用于同时定量天麻素、parishin A、parishin B、parishin C、和 parishin E。使用一步蛋白质沉淀法从血浆中提取了五种化合物。色谱分离在 C18 柱上实现，梯度流动相包含乙腈和 0.05% 甲酸。在多反应监测模式下使用负电喷雾电离进行检测。这种新方法通过使用最少的样品净化程序和更短的分析运行时间来最大化分析通量。该方法对浓度范围内的五种化合物 (r2 > 0.995) 表现出良好的线性。parishin A、parishin B、parishin C 和 parishin E 的定量下限 (LLOQ) 为 1.37 ng/mL，天麻素为 10 ng/mL。然后对该方法进行了日内和日间精密度、准确度、基质效应、提取回收率和稳定性的充分验证。这种经过验证的方法已成功应用于大鼠口服天麻提取物后的药代动力学研究。本研究可为天麻药理机制的临床应用和解释提供一定的指导。图形摘要图形摘要 萃取回收率高，稳定性好。这种经过验证的方法已成功应用于大鼠口服天麻提取物后的药代动力学研究。本研究可为天麻药理机制的临床应用和解释提供一定的指导。图形摘要图形摘要 萃取回收率高，稳定性好。这种经过验证的方法已成功应用于大鼠口服天麻提取物后的药代动力学研究。本研究可为天麻药理机制的临床应用和解释提供一定的指导。图形摘要图形摘要