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Role of phototherapy, BAX gene expression in hyperbilirubinemia development in full-term neonates
Egyptian Journal of Medical Human Genetics Pub Date : 2020-01-31 , DOI: 10.1186/s43042-019-0037-y
Mohamed farouk Afifi , Abdel Hakeem Abdel Mohsen , Emad Abdel Naeem , Marwa Ibrahem Abdel razic

Phototherapy is the main therapeutic interference for neonatal hyperbilirubinemia used to escape an exchange transfusion and to decrease the risk of bilirubin-induced encephalopathy (kernikterus). Phototherapy has an oxidative effect on cell components and cell membranes by enhancing peroxidation of lipid and damage to DNA. Many genes function as apoptosis regulatory genes. Examples of these genes involve the BCL2 gene as an anti-apoptotic oncogene, and the BAX gene which is a promoter of apoptosis. We aimed to evaluate the effect of phototherapy on expression of BAX and Bcl2 genes in hyperbilirubinemic full-term neonates. Eighteen full-term neonates with indirect hyperbilirubinemia who received phototherapy for 24 h were enrolled as a study group and nine apparently healthy full-term neonates with a normal serum bilirubin level were included as a control group. Assessment of the anti-apoptotic effect(s) of BCL2 and the pro-apoptotic effect(s) of (Bax) genes was achieved by quantitative assay of their products (BCL2 and BAX proteins) by ELISA assay after phototherapy. Significant decrease in the bcl2 (p < 0.001) and increase in Bax protein (p < 0.001) serum levels after phototherapy in hyperbilirubinemic full-term neonates. Hyperbilirubinemia has no apoptotic influence, while phototherapy induces apoptosis in the peripheral blood of hyperbilirubinemic full-term infants.

中文翻译:

光疗、BAX 基因表达在足月新生儿高胆红素血症发展中的作用

光疗是新生儿高胆红素血症的主要治疗干预措施,用于避免换血和降低胆红素诱发性脑病 (kernikterus) 的风险。光疗通过增强脂质过氧化和对 DNA 的损伤,对细胞成分和细胞膜产生氧化作用。许多基因起凋亡调控基因的作用。这些基因的例子包括作为抗细胞凋亡癌基因的BCL2基因和作为细胞凋亡启动子的BAX基因。我们旨在评估光疗对高胆红素血症足月新生儿 BAX 和 Bcl2 基因表达的影响。18 名接受光疗 24 h 的间接高胆红素血症足月新生儿作为研究组,9 名血清胆红素水平正常的表观健康足月新生儿作为对照组。BCL2 的抗凋亡作用和 (Bax) 基因的促凋亡作用的评估是通过光疗后通过 ELISA 测定对其产物(BCL2 和 BAX 蛋白)进行定量测定来实现的。高胆红素血症足月新生儿光疗后 bcl2 显着降低 (p < 0.001) 和 Bax 蛋白增加 (p < 0.001) 血清水平。高胆红素血症不影响细胞凋亡,而光疗可诱导高胆红素血症足月儿外周血细胞凋亡。BCL2 的抗凋亡作用和 (Bax) 基因的促凋亡作用的评估是通过光疗后通过 ELISA 测定对其产物(BCL2 和 BAX 蛋白)的定量测​​定来实现的。高胆红素血症足月新生儿光疗后 bcl2 显着降低 (p < 0.001) 和 Bax 蛋白增加 (p < 0.001) 血清水平。高胆红素血症不影响细胞凋亡,而光疗可诱导高胆红素血症足月儿外周血细胞凋亡。BCL2 的抗凋亡作用和 (Bax) 基因的促凋亡作用的评估是通过光疗后通过 ELISA 测定对其产物(BCL2 和 BAX 蛋白)的定量测​​定来实现的。高胆红素血症足月新生儿光疗后 bcl2 显着降低 (p < 0.001) 和 Bax 蛋白增加 (p < 0.001) 血清水平。高胆红素血症不影响细胞凋亡,而光疗可诱导高胆红素血症足月儿外周血细胞凋亡。
更新日期:2020-01-31
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