Stem bark of Anogeissus latifolia Roxb (family: Combretaceae) is used traditionally and ethnomedicinally to alleviate pain, inflammation and fever conditions. The current study investigates the analgesic, anti-inflammatory and antipyretic activities of ethanolic extract of stem bark of Anogeissus latifolia Roxb. The HPLC studies were carried out to standardize the stem bark ethanolic extract of Anogeissus latifolia (ALEE) using ellagic acid as a marker. ALEE was screened for analgesic (formalin-induced pain and acetic acid induced writhing) and anti-inflammatory (formalin and carrageenan-induced paw oedema) activities in Wistar rats. Before 1 h of acetic acid or formalin or carrageenan injection, rats were orally fed with ALEE (100, 200 and 400 mg/kg), Aspirin (100 mg/kg) or Indomethacin (10 mg/kg). Antipyretic effect was studied in brewer’s yeast-induced pyrexia model in rats using Paracetamol (100 mg/kg) as a standard drug. HPLC analysis of ALEE revealed the presence of ellagic acid. ALEE treatment (200 and 400 mg/kg) significantly inhibited pain response in both models. ALEE treatments prevented the raise of paw volume in both in-vivo models with percent inhibition of 44.40 and 46.21, respectively at 5 h. ALEE also showed a significant reduction of yeast-induced pyrexia till 4 h of treatment. ALEE exhibited analgesic, anti-inflammatory and antipyretic property in experimental models and validates traditional use of ALEE in pain, inflammation and fever.

中文翻译：

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干树皮的乙醇提取物的镇痛，抗炎及解热活动榆绿木叶冬青的罗

Anogeissus latifolia Roxb（家族：Combretaceae）的茎皮在传统上和民族学上用于缓解疼痛，炎症和发烧症状。当前的研究调查了无叶金合欢（Anogeissus latifolia Roxb）茎皮乙醇提取物的镇痛，抗炎和解热活性。进行了HPLC研究，以鞣花酸为标记物，标准化了无叶金线莲（ALEE）的茎皮乙醇提取物。在Wistar大鼠中，针对ALEE的镇痛（福尔马林引起的疼痛和乙酸引起的扭动）和抗炎（福尔马林和角叉菜胶引起的爪水肿）活性进行了筛选。在注射乙酸或福尔马林或角叉菜胶1小时之前，给大鼠口服ALEE（100、200和400 mg / kg），阿司匹林（100 mg / kg）或消炎痛（10 mg / kg）。使用扑热息痛（100 mg / kg）作为标准药物，在啤酒酵母诱导的大鼠发热模型中研究了解热作用。ALE的HPLC分析表明鞣花酸的存在。在两种模型中，ALEE治疗（200和400 mg / kg）均显着抑制了疼痛反应。ALEE治疗在两个体内模型中均阻止了爪子体积的增加，分别在5 h抑制百分比分别为44.40和46.21。ALEE还显示，直至治疗4小时，酵母菌引起的发热明显减少。ALEE在实验模型中显示出止痛，消炎和解热的特性，并验证了ALEE在疼痛，发炎和发烧中的传统用法。在两种模型中，ALEE治疗（200和400 mg / kg）均显着抑制了疼痛反应。ALEE治疗在两个体内模型中均阻止了爪子体积的增加，分别在5 h抑制百分比分别为44.40和46.21。ALEE还显示，直至治疗4小时，酵母菌引起的发热明显减少。ALEE在实验模型中显示出止痛，消炎和解热的特性，并验证了ALEE在疼痛，发炎和发烧中的传统用法。在两种模型中，ALEE治疗（200和400 mg / kg）均显着抑制了疼痛反应。ALEE治疗在两个体内模型中均阻止了爪子体积的增加，分别在5 h抑制百分比分别为44.40和46.21。ALEE还显示，直至治疗4小时，酵母菌引起的发热明显减少。ALEE在实验模型中显示出止痛，消炎和解热的特性，并验证了ALEE在疼痛，发炎和发烧中的传统用法。