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Evidence of multi-functional peptide activity: potential role of KT2 and RT2 for anti-inflammatory, anti-oxidative stress, and anti-apoptosis properties
Applied Biological Chemistry ( IF 3.2 ) Pub Date : 2020-01-21 , DOI: 10.1186/s13765-019-0488-3
Wisarut Payoungkiattikun , Anupong Joompang , Suyanee Thongchot , Boonpob Nowichai , Nisachon Jangpromma , Sompong Klaynongsruang

Although several explications of anti-inflammatory therapeutic substances for treating inflammatory-related diseases have been broadly discussed within the last few decades, peptide-based compounds display the potential to be novel inflammation treatment agents. Here, we evaluated the anti-inflammatory activity and other inflammation-associated activities, including anti-oxidative stress and anti-apoptosis properties, of the cationic peptides KT2 and RT2. Nitric oxide (NO) and other inflammatory markers were evaluated in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells co-incubated with peptides. The levels of interrelated gene and protein expressions were quantified. Peptides formed complexes with LPS and displayed anti-inflammatory properties by reducing NO and pro-inflammatory cytokine production in inflamed RAW 264.7 cells. These peptides also exhibit a strong suppressive effect on mRNA expression levels of inducible nitric oxide synthase, tumor necrosis factor-α, signal transducer and activator of transcription 1, c-Jun N-terminal protein kinase (JNK)-1, nuclear factor kappa B (NF-κB), and p38 mitogen-activated protein kinase (MAPK), which affects the decay of phosphorylated JNK-1, p38 MAPK, and NF-κB p65 protein expression. Both peptides induce up-regulation of anti-inflammatory mRNA and protein expression levels of extracellular signal-regulated kinase and mRNA expression levels of MAPK phosphatase-1. Also, the production of reactive oxygen species was observed to be markedly reduced. Furthermore, peptides exhibited an anti-apoptotic property. To our knowledge, this is the first report of the multi-functional peptides KT2 and RT2 exerting broad biological activity related to anti-inflammatory effects. These peptides have potential for delivering a medical method for the handling of inflammation-related diseases.

中文翻译:

多功能肽活性的证据:KT2和RT2在抗炎,抗氧化应激和抗凋亡特性中的潜在作用

尽管在过去的几十年中已经广泛讨论了用于治疗炎症相关疾病的抗炎治疗物质的几种说明,但是基于肽的化合物显示出成为新型炎症治疗剂的潜力。在这里,我们评估了阳离子肽KT2和RT2的抗炎活性和其他与炎症相关的活性,包括抗氧化应激和抗凋亡特性。在与多肽共同孵育的脂多糖(LPS)刺激的RAW 264.7细胞中评估了一氧化氮(NO)和其他炎症标记。定量相关基因和蛋白质表达的水平。肽与LPS形成复合物,并通过减少发炎的RAW 264.7细胞中的NO和促炎性细胞因子产生而显示出抗炎特性。这些肽还对诱导型一氧化氮合酶,肿瘤坏死因子-α,信号转导子和转录激活因子1,c-Jun N端蛋白激酶(JNK)-1,核因子kappa B的mRNA表达水平具有强烈的抑制作用。 (NF-κB)和p38丝裂原活化蛋白激酶(MAPK),这会影响磷酸化JNK-1,p38 MAPK和NF-κBp65蛋白表达的衰减。两种肽均可诱导抗炎mRNA和细胞外信号调节激酶的蛋白表达水平以及MAPK磷酸酶-1的mRNA表达水平的上调。另外,观察到活性氧的产生显着减少。此外,肽表现出抗凋亡特性。据我们所知,这是多功能肽KT2和RT2具有与抗炎作用相关的广泛生物学活性的首次报道。这些肽具有提供用于治疗炎症相关疾病的医学方法的潜力。
更新日期:2020-01-21
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