Hemocyanin, the predominant protein in the hemolymph of crustaceans, was isolated from the plasma of the giant freshwater prawn Macrobrachium rosenbergii (De Man, 1879) and investigated, with its separated proteins MrHc1, 2, 3, and 4 (60, 114, 50, and 325 kDa, respectively) for hemagglutination (HA) activity. Hemocyanin against erythrocytes from human A, B, and O blood groups, mouse, rat sheep, water buffalo, and rabbit showed HA titer values higher than plasma. Of the four proteins isolated from hemocyanin, MrHc1 alone appeared to agglutinate the tested erythrocytes. Evaluated specific hemagglutination activity was higher for hemocyanin when compared to plasma. Sugar binding ability with N-acetylated sugars observed for plasma was restricted to N-acetyl neuraminic acid for hemocyanin and MrHc1. Inhibition of hemagglutination (HAI) by bovine submaxillary mucin (BSM) further defined the agglutination ability of hemocyanin and MrHc1. The hemocyanin protein MrHc1 on matrix-assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI–TOF/MS) and search in MASCOT database showed homology to hypothetical protein of Branchiostoma floridae Hubbs, 1922 (Cephalochordata). The results clearly indicated that the structure of the hemocyanin had evolved binding sites for sugar or sugar-linkages independently lacking homology with other hemocyanin or lectin-like proteins. The immunogenicity of this hemocyanin probably has biomedical applications as carrier adjuvants, which needs further investigation.

中文翻译：

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淡水虾罗氏沼虾的血淋巴中血浆，血蓝蛋白和分离的血蓝蛋白的凝集（德曼，1879年）（十足目：Caridea：Palaemonidae）

从巨型淡水虾罗氏沼虾的血浆中分离出甲壳素血淋巴中的主要蛋白质血蓝蛋白。（De Man，1879），并用其分离的蛋白MrHc1、2、3和4（分别为60、114、50和325 kDa）研究了血凝（HA）活性。血红蛋白针对人A，B和O血型的红细胞，小鼠，大鼠绵羊，水牛和兔子的HA滴度值高于血浆。从血蓝蛋白分离出的四种蛋白质中，仅MrHc1似乎会凝集被测红细胞。与血浆相比，血红蛋白的评估比血凝活性更高。血浆中观察到的与N-乙酰化糖的糖结合能力仅限于N-乙酰神经氨酸的血蓝蛋白和MrHc1。牛下颌粘蛋白（BSM）对血凝（HAI）的抑制作用进一步定义了血蓝蛋白和MrHc1的凝集能力。佛罗里达科的枝条，1922年（Cephalochordata）。结果清楚地表明，血红蛋白的结构已经进化出糖或糖键的结合位点，这些位点独立地缺乏与其他血蓝蛋白或凝集素样蛋白的同源性。这种血蓝蛋白的免疫原性可能具有生物医学应用作为载体佐剂，需要进一步研究。