Plant proteinase inhibitors are among the promising biopesticides which are induced in plants tissues against the several Lepidoptera pests to inhibit digestive proteases. In this study, protein extracts of two nonhost plant seeds, Amaranthus retroflexus Linnaeus (Amaranthaceae) and Cuminum cyminum Linnaeus (Apiaceae), were examined on Helicoverpaarmigera (Hübner) (Lepidoptera: Noctuidae). The results obtained by using azocasein as a substrate showed that inhibitory activity of general proteases of the larvae fed on a diet incorporated with both inhibitors was dose dependent. Seed extracts of A. retroflexus and C. cyminum at the highest concentration showed that inhibition activities of chymotrypsin-like proteinase and trypsin-like proteinase were between 31–45% and 28–61%, respectively. Based on polyacrylamide gel electrophoresis, all of the proteinase isoforms, including those of A. retroflexus seed extracts, disappeared entirely, and only one band was detected in the seed extracts of C. cyminum. Larval mortality in the larvae fed on A. retroflexus and C. cyminum seed extracts was 56 ± 2.15 and 68 ± 2.23, respectively, but mortality in control (no seed protein extract) was 12 ± 2.34 individuals. Also, the life table parameters were affected significantly by A. retroflexus and C. cyminum protein seed extracts. Therefore, A. retroflexus and C. cyminum seed protein extracts showed inhibitory effect on H. armigera digestive proteinases and adverse effects on survival and fitness of the pest; hence, they could be introduced as a successful biopesticide in the near future.

植物蛋白酶抑制剂是在植物组织中针对几种鳞翅目害虫诱导的有希望的生物农药之一，以抑制消化蛋白酶。在这项研究中，两个非寄主植物种子的蛋白质提取物苋林奈（苋）和孜然林奈（伞形科），检查对棉铃虫棉铃虫（棉铃虫）（夜蛾）。通过使用偶氮酪蛋白作为底物获得的结果表明，在掺入两种抑制剂的饮食中喂食的幼虫的一般蛋白酶的抑制活性是剂量依赖性的。种子提取物反枝苋和C.孜然在最高浓度下，胰凝乳蛋白酶样蛋白酶和胰蛋白酶样蛋白酶的抑制活性分别在31–45％和28–61％之间。基于聚丙烯酰胺凝胶电泳中，所有的蛋白酶同种型，包括那些的反枝苋种子提取物，完全消失，而在种子提取物中仅检测到一个带C.芹。取食逆向弯曲杆菌和C.cyminum种子提取物的幼虫的幼虫死亡率分别为56±2.15和68±2.23，而对照（无种子蛋白提取物）的死亡率为12±2.34个人。此外，生命表参数进行了显著影响反枝苋和C.孜然蛋白种子提取物。因此，反枝苋和C.芹种子蛋白提取物显示出对抑制效果棉铃虫消化蛋白酶和对存活和害虫的健身不利影响; 因此，它们可以在不久的将来作为一种成功的生物农药引入。