Three-dimensional cultures have exciting potential to mimic aspects of healthy and diseased brain tissue to examine the role of physiological conditions on neural biomarkers, as well as disease onset and progression. Hypoxia is associated with oxidative stress, mitochondrial damage, and inflammation, key processes potentially involved in Alzheimer's and multiple sclerosis. We describe the use of an enzymatically-crosslinkable gelatin hydrogel system within a microfluidic device to explore the effects of hypoxia-induced oxidative stress on rat neuroglia, human astrocyte reactivity, and myelin production. This versatile platform offers new possibilities for drug discovery and modeling disease progression.

中文翻译：

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使用酶交联水凝胶对神经胶质细胞对缺氧诱导的氧化应激的反应

三维培养具有令人兴奋的潜力，可以模拟健康和患病脑组织的各个方面，以检查生理条件对神经生物标志物的作用，以及疾病的发生和进展。缺氧与氧化应激、线粒体损伤和炎症有关，这些关键过程可能与阿尔茨海默氏症和多发性硬化症有关。我们描述了在微流体装置中使用酶促交联明胶水凝胶系统来探索缺氧诱导的氧化应激对大鼠神经胶质细胞、人类星形胶质细胞反应性和髓鞘产生的影响。这个多功能平台为药物发现和疾病进展建模提供了新的可能性。