In recent years, electrochemiluminescence (ECL) nanosensing systems have undergone rapid development and made significant progress in ultrasensitive analysis and cell imaging. Because of the unique advantages of high selectivity, ultra-sensitivity, and good reproducibility, ECL nanosensors can open new paths for cancer diagnosis. With the development of ECL nanosensors, high-throughput analysis, visual detection and spatially resolved ECL imaging of single cells are being realized. The innovations of ECL nanosensors consist of electrochemical excitation, coreactant catalysis, light radiation and luminescence signal amplification, which involve several fields such as nanotechnology, catalysis, optics, and electrochemistry. The developments of ECL instruments also relate to imaging technology. Herein, we review the construction modes, sensing strategies and cancer diagnosis applications of ECL nanosenors. Firstly, the nano-components of the ECL sensing system are discussed. The construction and signal amplification methods of the nanosensing system are emphasized. Secondly, the high-efficiency cancer identification strategies are presented, including protein tumor marker detection, nucleic acid assay, cancer cell identification and exosome detection. The recent advances in representative examples of ECL nanosenors in cancer diagnosis are highlighted, including high-throughput ECL analysis, in situ assay, visual ECL detection, single-cell imaging diagnosis, and so on. Finally, the challenges are featured based on the recent development of the ECL nanosensing system in the clinical diagnosis. The ECL nanosensors provide effective and reliable analytical methods and open new paths for cancer diagnosis. It is noteworthy that the prospects of the ECL nanosensing system in clinical diagnosis are instructive to the developments of other nanosensor research.

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用于癌症诊断应用的电化学发光纳米传感器的最新发展。

近年来，电化学发光（ECL）纳米传感系统得到了快速发展，并在超灵敏分析和细胞成像中取得了重大进展。由于具有高选择性，超灵敏性和良好的可重复性的独特优势，ECL纳米传感器可以为癌症诊断开辟新的途径。随着ECL纳米传感器的发展，单细胞的高通量分析，视觉检测和空间分辨ECL成像正在实现。ECL纳米传感器的创新包括电化学激发，共反应物催化，光辐射和发光信号放大，涉及纳米技术，催化，光学和电化学等多个领域。ECL仪器的发展也与成像技术有关。在这里，我们回顾一下构建模式，ECL纳米传感器的传感策略和癌症诊断应用。首先，讨论了ECL传感系统的纳米组件。重点介绍了纳米传感系统的构建和信号放大方法。其次，提出了高效的癌症鉴定策略，包括蛋白质肿瘤标志物检测，核酸测定，癌细胞鉴定和外来体检测。着重介绍了ECL纳米传感器在癌症诊断中具有代表性的最新进展，包括高通量ECL分析，提出了高效的癌症鉴定策略，包括蛋白质肿瘤标记物检测，核酸测定，癌细胞鉴定和外来体检测。着重介绍了ECL纳米传感器在癌症诊断中具有代表性的最新进展，包括高通量ECL分析，提出了高效的癌症鉴定策略，包括蛋白质肿瘤标记物检测，核酸测定，癌细胞鉴定和外来体检测。着重介绍了ECL纳米传感器在癌症诊断中具有代表性的最新进展，包括高通量ECL分析，原位测定，视觉ECL检测，单细胞成像诊断等。最后，基于临床诊断中ECL纳米传感系统的最新发展，提出了挑战。ECL纳米传感器提供了有效而可靠的分析方法，为癌症诊断开辟了新途径。值得注意的是，ECL纳米传感系统在临床诊断中的前景对其他纳米传感器研究的发展具有指导意义。