A self-templated strategy was adopted to design hollow Co₃O₄/MO₃ (M = Mo, W) mixed-metal oxides via the Mo or W doping of ZIF-67, and subsequent pyrolysis under an atmosphere of air at a low temperature of 450 °C. The hollow Co₃O₄/MO₃ (M = Mo, W) mixed-metal oxides displayed tunable oxidase-like and peroxidase-like activities able to efficiently catalyze the oxidation of TMB to generate a deep blue color in the absence or presence of H₂O₂. Relative to that of the un-doped Co₃O₄, the oxidase mimic activity of the Mo-doped Co₃O₄ increased to 1.3 to 2.1-fold, while its peroxidase mimic activity increased to 7.1 to 19.9-fold, depending on different Mo doping amounts. The oxidase mimic activity of the W-doped Co₃O₄ increased to 2.1 to 2.3-fold, while its peroxidase mimic activity increased to 4.8 to 5.9-fold, depending on the different W doping amounts. The Mo- and W-doped Co₃O₄ nanohybrid exhibited both higher O₂ and H₂O₂ activating capability, and their H₂O₂ activating capacity was superior to the O₂ activating capability. Furthermore, the Mo- and W-doped Co₃O₄ nanohybrids exhibited similar O₂ activating abilities, while the Mo-doped one displayed a higher H₂O₂ activating capability than the W-doped one. The discrepant peroxidase-like nature of Mo- and W-doped Co₃O₄ nanohybrids is likely attributed to their different catalytic mechanisms. The peroxidase-like activity of Mo-doped Co₃O₄ is highly related to the ˙OH free radical, while that of W-doped Co₃O₄ is likely ascribed to the electron transfer between TMB and H₂O₂. The K_m values of Co₃O₄/MoO₃ for TMB and H₂O₂ were 0.0352 mM and 0.134 mM, which were 3.2- and 1.9-fold lower than that of pure Co₃O₄, respectively. A Co₃O₄/MoO₃-based colorimetric platform was developed for the determination of H₂O₂ in the 0.1–200 μM range, with a limit of detection of 0.08 μM (3σ). Based on the thiocholine (TCh) inhibition of the excellent peroxidase-like activity of Co₃O₄/MoO₃ and the TCh generation via acetylcholinesterase (AChE) catalyzed hydrolysis of acetylthiocholine chloride (ATCh), the colorimetric platform was extended to screen AChE activity and its inhibitor.

中文翻译：

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一种可调节的双功能空心Co3O4 / MO3（M = Mo，W）混合金属氧化物纳米酶，用于感测H2O2和筛选乙酰胆碱酯酶活性及其抑制剂。

一种自模板策略获得通过设计中空钴₃ ö ₄ / MO ₃（M =钼，钨）的混合金属氧化物通过空气的气氛下在低的Mo或ZIF-67 W的掺杂，和随后的热解温度为450°C。中空的Co ₃ O ₄ / MO ₃（M = Mo，W）混合金属氧化物表现出可调节的氧化酶样和过氧化物酶样活性，能够有效地催化TMB的氧化，从而在不存在或不存在TMB的情况下产生深蓝色。 H ₂ O ₂。相对于未掺杂的Co ₃ O ₄，Mo掺杂的Co ₃ O的氧化酶模拟活性₄增至1.3至2.1倍，而其过氧化物酶模拟活性增至7.1至19.9倍，具体取决于不同的Mo掺杂量。掺杂W的Co ₃ O ₄的氧化酶模拟活性增加至2.1至2.3倍，而其过氧化物酶模拟活性增加至4.8至5.9倍，这取决于不同的W掺杂量。Mo和W掺杂的Co ₃ O ₄纳米杂化物同时具有较高的O ₂和H ₂ O ₂活化能力，并且它们的H ₂ O ₂活化能力优于O ₂活化能力。此外，Mo和W掺杂的Co ₃ O_4种纳米杂化物表现出相似的O ₂活化能力，而Mo掺杂的纳米杂化物表现出比W掺杂的更高的H ₂ O ₂活化能力。Mo和W掺杂的Co ₃ O ₄纳米杂化物的不同的过氧化物酶样性质可能归因于它们不同的催化机理。Mo掺杂的Co ₃ O ₄的过氧化物酶活性与˙OH自由基高度相关，而W掺杂的Co ₃ O ₄的过氧化物酶活性可能归因于TMB和H ₂ O ₂之间的电子转移。Co ₃的K _m值TMB和H ₂ O _2的O ₄ / MoO ₃分别为0.0352 mM和0.134 mM，分别比纯Co ₃ O _4的低_3.2倍和1.9倍。开发了基于Co ₃ O ₄ / MoO ₃的比色平台，用于测定0.1-200μM范围内的H ₂ O ₂，检出限为0.08μM（3σ）。基于Co的优异的过氧化物酶样活性的硫代胆碱（TCH）抑制₃ ö ₄ /的MoO ₃和TCH代经由 乙酰胆碱酯酶（AChE）催化乙酰硫代胆碱氯化物（ATCh）的水解，比色平台得以扩展以筛选AChE活性及其抑制剂。