Veterinary pathology tissue banks are valuable resources for genetic studies. However, limited data exist as to whether quality DNA can be extracted from these tissues for use in canine genotyping studies. We extracted DNA from 44 formalin-fixed, paraffin-embedded (FFPE) tissue blocks from dogs; 9 of these dogs had DNA available from whole blood samples that had been banked. We genotyped DNA from 30 of 44 tissue blocks and 9 whole blood samples on the Illumina CanineHD BeadChip; DNA quality was insufficient in 14 of 44 samples from tissue blocks. There was significant correlation between the 260/280 ratio and single-nucleotide variation (SNV) call rate (p = 0.0276; r² = 0.162); 23 of 30 samples from FFPE were genotyped with > 65% call rates. Median pairwise identical-by-state (IBS) analysis was 0.99 in 8 pairs of dogs with call rates > 65%. Neither age of tissue block nor specific tissue types were associated with significant differences in DNA concentration, 260/280 ratio, or SNV call rate. DNA extracted from tissue blocks can have variable quality, although comparable levels of homozygosity suggest that extracts from FFPE with call rates > 65% might provide similar results to samples from whole blood when analyzed on the Illumina CanineHD BeadChip.

兽医病理组织库是遗传研究的宝贵资源。但是，关于是否可以从这些组织中提取高质量的DNA用于犬类基因分型研究的数据有限。我们从狗的44个福尔马林固定，石蜡包埋（FFPE）组织块中提取了DNA；这些狗中有9只具有从已存储的全血样本中获得的DNA。我们在Illumina CanineHD BeadChip上对44个组织块中的30个和9个全血样本中的DNA进行了基因分型；在来自组织块的44个样本中，有14个样本的DNA质量不足。260/280比率与单核苷酸变异（SNV）检出率之间存在显着相关性（p = 0.0276; r ²= 0.162）；对来自FFPE的30个样本中的23个进行了基因分型，检出率> 65％。在八对成对的狗中，成对的状态相同（IBS）分析为0.99，呼叫率> 65％。组织阻塞的年龄和特定组织类型均与DNA浓度，260/280比或SNV检出率的显着差异无关。从组织块提取的DNA可能具有可变的质量，尽管纯合度的可比水平表明，在Illumina CanineHD BeadChip上进行分析时，检出率> 65％的FFPE提取物可能提供与全血样品相似的结果。