Obesity, defined as a body mass index of 30 kg/m² or above, has increased considerably in incidence and frequency within the United States and globally. Associated comorbidities including cardiovascular disease, type 2 diabetes mellitus, metabolic syndrome, and nonalcoholic fatty liver disease have led to a focus on the mechanisms promoting the prevention and treatment of obesity. Commonly utilized in vitro models employ human or mouse preadipocyte cell lines in a 2-dimensional (2D) format. Due to the structural, biochemical, and biological limitations of these models, increased attention has been placed on “organ on a chip” technologies for a 3-dimensional (3D) culture. Herein, we describe a method employing cryopreserved primary human stromal vascular fraction (SVF) cells and a human blood product-derived biological scaffold to create a 3D adipose depot in vitro. The “fat-on-chip” 3D cultures have been validated relative to 2D cultures based on proliferation, flow cytometry, adipogenic differentiation, confocal microscopy/immunofluorescence, and functional assays (adipokine secretion, glucose uptake, and lipolysis). Thus, the in vitro culture system demonstrates the critical characteristics required for a humanized 3D white adipose tissue (WAT) model.

中文翻译：

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二维和三维文化中的人类脂肪来源的细胞：体外脂肪构建体的功能验证。

肥胖定义为30 kg / m ²或更高的身体质量指数，在美国和全球范围内，其发病率和发生率已大大增加。包括心血管疾病，2型糖尿病，代谢综合症和非酒精性脂肪肝在内的合并症已引起人们对肥胖预防和治疗机制的关注。体外常用这些模型采用二维（2D）格式的人类或小鼠前脂肪细胞系。由于这些模型的结构，生化和生物学方面的局限性，人们越来越关注3D（3D）培养的“芯片上的器官”技术。在这里，我们描述了一种方法，该方法利用冷冻保存的原代人基质血管分数（SVF）细胞和人血制品来源的生物支架在体外创建3D脂肪库。已基于增殖，流式细胞仪，成脂分化，共聚焦显微镜/免疫荧光和功能测定（脂肪因子分泌，葡萄糖摄取和脂解）相对于2D培养物对“芯片上脂肪” 3D培养物进行了验证。因此，体外 培养系统展示了人性化3D白色脂肪组织（WAT）模型所需的关键特性。