Reversibly cross-linked adaptable hydrogels show great advantages in tissue engineering. The dynamic adaptable networks can overcome three-dimensional (3D) physical restrictions to enable normal cellular functions without hydrogel degradation. However, because of the dynamic reversibility, adaptable hydrogels typically exhibit weak mechanical properties and rapid erosion behaviors. Herein, we develop a facile strategy to prepare stable adaptable hydrogels using dynamic covalent chemistry, stable covalent chemistry, and the interpenetrating polymeric network (IPN) strategy. The developed IPN HA/γ-PGA adaptable hydrogels have stable structures, good mechanical properties, enzymatic degradability, and injectability. Compression test indicates that although containing 95% water, the IPN HA/γ-PGA adaptable hydrogels can suffer more than 85% compressive strain and show a fast shape recovery capacity and good antifatigue ability. Benefitting from the good cytocompatibility of functionalized HA and γ-PGA and the mild preparation process of IPN HA/γ-PGA adaptable hydrogels, NIH 3T3 cells can tolerate the 3D encapsulation process and show high cell viability. Therefore, owing to their desirable properties, the developed HA/γ-PGA adaptable hydrogels have great potential applications for load-bearing tissue engineering.

中文翻译：

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基于透明质酸和聚（γ-谷氨酸）的共价适应性水凝胶用于潜在的承重组织工程

可逆交联的适应性水凝胶在组织工程中显示出巨大的优势。动态自适应网络可以克服三维 (3D) 物理限制，以实现正常的细胞功能而不会发生水凝胶降解。然而，由于动态可逆性，适应性水凝胶通常表现出较弱的机械性能和快速的侵蚀行为。在此，我们开发了一种简便的策略，使用动态共价化学、稳定的共价化学和互穿聚合物网络 (IPN) 策略制备稳定的适应性水凝胶。开发的IPN HA/γ-PGA适应性水凝胶具有结构稳定、力学性能好、酶促降解性和可注射性等优点。压缩试验表明，虽然含有 95% 的水，IPN HA/γ-PGA适应性水凝胶可承受85%以上的压缩应变，具有快速的形状恢复能力和良好的抗疲劳能力。得益于功能化 HA 和 γ-PGA 良好的细胞相容性以及 IPN HA/γ-PGA 适应性水凝胶的温和制备过程，NIH 3T3 细胞可以耐受 3D 封装过程并显示出高细胞活力。因此，由于其理想的特性，所开发的HA/γ-PGA适应性水凝胶在承重组织工程中具有巨大的潜在应用。