Seed longevity is a polygenic trait of relevance for agriculture and for understanding the effect of environment on the ageing of biological systems. In order to identify novel longevity genes, we have phenotyped the natural variation of 270 ecotypes of the model plant, Arabidopsis thaliana, for natural ageing and for three accelerated ageing methods. Genome‐wide analysis, using publicly available single‐nucleotide polymorphisms (SNPs) data sets, identified multiple genomic regions associated with variation in seed longevity. Reverse genetics of 20 candidate genes in Columbia ecotype resulted in seven genes positive for seed longevity (PSAD1, SSLEA, SSTPR, DHAR1, CYP86A8, MYB47 and SPCH) and five negative ones (RBOHD, RBOHE, RBOHF, KNAT7 and SEP3). In this uniform genetic background, natural and accelerated ageing methods provided similar results for seed‐longevity in knock‐out mutants. The NADPH oxidases (RBOHs), the dehydroascorbate reductase (DHAR1) and the photosystem I subunit (PSAD1) highlight the important role of oxidative stress on seed ageing. The cytochrome P‐450 hydroxylase, CYP86A8, and the transcription factors, MYB47, KNAT7 and SEP3, support the protecting role of the seed coat during seed ageing.

中文翻译：

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通过全基因组关联研究和反向遗传学鉴定与氧化胁迫和种皮有关的新型种子寿命基因。

种子寿命是与农业相关的多基因性状，对于了解环境对生物系统衰老的影响也是如此。为了鉴定新的长寿基因，我们对自然老化和三种加速老化方法的模型植物拟南芥的270种生态型的自然变异进行了表型分析。全基因组分析使用公开可用的单核苷酸多态性（SNP）数据集，确定了多个与种子寿命变化相关的基因组区域。哥伦比亚生态型的20个候选基因的反向遗传学产生了7个种子寿命阳性的基因（PSAD1，SSLEA，SSTPR，DHAR1，CYP86A8，MYB47和SPCH）和五个负面的（RBOHD，RBOHE，RBOHF，KNAT7和SEP3）。在这种统一的遗传背景下，自然和加速衰老方法为敲除突变体的种子寿命提供了相似的结果。NADPH氧化酶（RBOHs），脱氢抗坏血酸还原酶（DHAR1）和光系统I亚基（PSAD1）突出了氧化胁迫对种子老化的重要作用。细胞色素P-450羟化酶CYP86A8和转录因子MYB47，KNAT7和SEP3在种子老化过程中支持种皮的保护作用。