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Overexpression of improved EPSPS gene results in field level glyphosate tolerance and higher grain yield in rice.
Plant Biotechnology Journal ( IF 13.8 ) Pub Date : 2020-06-09 , DOI: 10.1111/pbi.13428
V Mohan Murali Achary 1 , Vijay Sheri 1 , Mrinalini Manna 1 , Varakumar Panditi 1 , Bhabesh Borphukan 1 , Babu Ram 1 , Aakrati Agarwal 1 , Dhirendra Fartyal 1 , Deepa Teotia 1 , Shyam Kumar Masakapalli 2 , Pawan K Agrawal 3 , Malireddy K Reddy 1
Affiliation  

Glyphosate is a popular, systemic, broad‐spectrum herbicide used in modern agriculture. Being a structural analog of phosphoenolpyruvate (PEP), it inhibits 5‐enolpyruvylshikimate 3‐phosphate synthase (EPSPS) which is responsible for the biosynthesis of aromatic amino acids and various aromatic secondary metabolites. Taking a lead from glyphosate‐resistant weeds, two mutant variants of the rice EPSPS gene were developed by amino acid substitution (T173I + P177S; TIPS‐OsEPSPS and G172A + T173I + P177S; GATIPS‐OsEPSPS). These mutated EPSPS genes were overexpressed in rice under the control of either native EPSPS or constitutive promoters (maize ubiquitin [ZmUbi] promoter). The overexpression of TIPS‐OsEPSPS under the control of the ZmUbi promoter resulted in higher tolerance to glyphosate (up to threefold of the recommended dose) without affecting the fitness and related agronomic traits of plants in both controlled and field conditions. Furthermore, such rice lines produced 17%–19% more grains compared to the wild type (WT) in the absence of glyphosate application and the phenylalanine and tryptophan contents in the transgenic seeds were found to be significantly higher in comparison with WT seeds. Our results also revealed that the native promoter guided expression of modified EPSPS genes did not significantly improve the glyphosate tolerance. The present study describing the introduction of a crop‐specific TIPS mutation in class I aroA gene of rice and its overexpression have potential to substantially improve the yield and field level glyphosate tolerance in rice. This is the first report to observe that the EPSPS has role to play in improving grain yield of rice.

中文翻译:

改善的EPSPS基因的过表达导致田间水平的草甘膦耐受性和水稻更高的籽粒产量。

草甘膦是一种广泛用于现代农业的系统性广谱除草剂。作为磷酸烯醇丙酮酸(PEP)的结构类似物,它抑制5-烯醇丙酮酸shi草酸酯3-磷酸合酶(EPSPS),后者负责芳香族氨基酸和各种芳香次级代谢产物的生物合成。以抗草甘膦的杂草为先导,通过氨基酸置换开发了水稻EPSPS基因的两个突变体(T173I + P177S;TIPS-OsEPSPS和G172A + T173I + P177S;GATIPS-OsEPSPS)。这些突变的EPSPS基因在天然EPSPS或组成型启动子(玉米泛素[ ZmUbi ]启动子)的控制下在水稻中过表达。过分表达ZmUbi启动子控制下的TIPS-OsEPSPS导致对草甘膦的耐受性更高(最高为推荐剂量的三倍),而在受控和田间条件下均不影响植物的适应性和相关农艺性状。此外,在不使用草甘膦的情况下,这种水稻品系的谷物比野生型(WT)多出17%–19%,并且发现转基因种子中的苯丙氨酸和色氨酸含量明显高于野生型。我们的结果还表明,天然启动子指导的修饰EPSPS基因表达不能显着提高草甘膦的耐受性。本研究描述了在I类aroA中引入作物特异性TIPS突变水稻基因及其过表达可能会大大提高水稻的产量和田间水平对草甘膦的耐受性。这是第一份观察到EPSPS在提高水稻籽粒产量方面发挥作用的报告。
更新日期:2020-06-09
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