Using prediction and experimental determination, we compared the RNA‐editing sites in chloroplast protein‐coding genes in an early‐maturity mutant (KFJT‐1) induced by carbon‐ion beam irradiation in sweet sorghum and its wild type (KFJT‐CK). Twenty‐two and 20 editing sites were identified in the KFJT‐CK and KFJT‐1 chloroplast, respectively, and the differential editing sites between KFJT‐CK and KFJT‐1 were mainly in mat, ndh and rpo genes. Furthermore, mutations in RNA‐editing sites in KFJT‐1 affected the structure of the encoded protein, which implying that RNA‐editing events might influence the function of essential genes. Finally, a comparative analysis of the RNA‐editing events between KFJT‐1 and other 10 Poaceae species revealed that the RNA‐editing sites in matK‐1002, rpoC1‐1163 and rpoC2‐242 were unique to KFJT‐1. This suggested that RNA‐editing events due to carbon‐ion beam irradiation affected the structure of the encode proteins, thereby influenced their biological functions. This study is the first to report on the variation in chloroplast RNA‐editing patterns induced by carbon‐ion beams irradiation, and its effect on the chloroplast in KFJT‐1 at the post‐transcriptional level.

中文翻译：

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碳离子束诱导甜高粱早熟突变体叶绿体蛋白编码基因RNA编辑位点的变化

通过预测和实验确定，我们比较了碳离子束辐照甜高粱及其野生型（KFJT-CK）诱导的早熟突变体（KFJT-1）中叶绿体蛋白编码基因中的RNA编辑位点。在KFJT-CK和KFJT-1叶绿体中分别鉴定出22个和20个编辑位点，KFJT-CK和KFJT-1之间的差异编辑位点主要在mat，ndh和rpo中。基因。此外，KFJT-1中RNA编辑位点的突变影响了编码蛋白的结构，这意味着RNA编辑事件可能会影响必需基因的功能。最后，KFJT-1等10种禾本科之间的RNA编辑活动的比较分析表明：在RNA编辑位点matK基因-1002，rpoC1 -1163和rpoC2‐242是KFJT‐1独有的。这表明由于碳离子束辐照引起的RNA编辑事件影响了编码蛋白的结构，从而影响了它们的生物学功能。这项研究首次报道了碳离子束辐照诱导的叶绿体RNA编辑模式的变化，及其在转录后水平对KFJT-1中叶绿体的影响。