Dioscorea nipponica Makino has been used for the treatment of chronic bronchitis, rheumatoid arthritis, cough, and asthma. Several studies have established the antitumor effect of D. nipponica Makino extract (DNE). However, no investigations have considered the antimetastatic potential of DNE in cervical cancer cells. The present study examined the effects of DNE on cervical cancer cells treated with 12‐O‐tetradecanoylphorbol‐13‐acetate and characterized the possible molecular mechanisms. MTT assay results indicated that DNE exhibited very low cytotoxicity, and DNE significantly reduced the invasion and migration abilities of cervical cancer cells. Gelatin zymography analysis revealed that DNE significantly inhibited matrix metalloproteinase‐9 (MMP‐9) activity. Reverse transcription‐polymerase chain reaction assay results revealed that DNE treatment inhibited the MMP‐9 mRNA levels of HeLa and SiHa cells. Western blot results revealed that DNE significantly diminished the ERK1/2 phosphorylation. In conclusion, we revealed that the antimetastatic effects of DNE on cervical cancer cells are due to its inhibition of MMP‐9 expression through the ERK1/2 pathway.

中文翻译：

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Dioscorea nipponica Makino 通过抑制人宫颈癌细胞中的基质金属蛋白酶 9 抑制 TPA 诱导的迁移和侵袭

Dioscorea nipponica Makino 已用于治疗慢性支气管炎、类风湿性关节炎、咳嗽和哮喘。几项研究已经确定了日本 D. nipponica Makino 提取物 (DNE) 的抗肿瘤作用。然而，没有研究考虑过 DNE 在宫颈癌细胞中的抗转移潜力。本研究检查了 DNE 对 12-O-十四烷酰佛波醇-13-乙酸酯处理的宫颈癌细胞的影响，并表征了可能的分子机制。MTT检测结果表明DNE表现出极低的细胞毒性，并且DNE显着降低宫颈癌细胞的侵袭和迁移能力。明胶酶谱分析显示 DNE 显着抑制基质金属蛋白酶 9 (MMP-9) 活性。逆转录聚合酶链反应测定结果显示，DNE 处理抑制了 HeLa 和 SiHa 细胞的 MMP-9 mRNA 水平。蛋白质印迹结果显示 DNE 显着减少了 ERK1/2 磷酸化。总之，我们揭示了 DNE 对宫颈癌细胞的抗转移作用是由于其通过 ERK1/2 途径抑制 MMP-9 的表达。