Systemic immune responses in cancer patients are of tremendous importance, both to advance understanding of disease mechanisms and for development of new diagnostic testing. Minimal published information is available on the systemic cytokine response in canine osteosarcoma (OS) patients. The goal of this study was to investigate serum cytokine alterations present in OS patients at the time of diagnosis. Serum samples from 22 canine OS patients at the time of diagnosis and 18 healthy control dogs were evaluated via multiplex immunoassay for 14 analytes. Significant increases in serum interleukin-8 (IL-8) and interleukin-12p40 (IL-12p40) concentrations were found in OS patients when compared to healthy controls. The results correlate with several published studies on serum cytokine alterations in human OS patients. These data add to the growing body of knowledge on immunologic alterations in OS, including potential immunomodulatory therapy of canine patients, and support future studies on serum cytokine testing to investigate diagnostic and prognostic utility.

癌症患者的全身免疫应答对于提高对疾病机制的了解和开发新的诊断检测均至关重要。有关犬骨肉瘤（OS）患者的全身细胞因子反应的公开信息很少。这项研究的目的是调查诊断时OS患者中存在的血清细胞因子改变。通过多重免疫分析对14种分析物评估了22例犬OS诊断患者和18只健康对照犬的血清样本。与健康对照组相比，在OS患者中发现血清白细胞介素8（IL-8）和白细胞介素12p40（IL-12p40）浓度显着增加。该结果与关于人类OS患者血清细胞因子改变的几项已发表研究相关。