Purpose

Mycoplasma capricolum subsp. capripneumoniae (Mccp) causes a severe, usually fatal disease in goats known as Contagious Caprine Pleuropneumonia (CCPP). CCPP is listed by OIE as a notifiable animal diseases, causing economic losses in terms of high morbidity and mortality. Thus far, very limited information is available on the molecular characterization of the unique Mccp strains prevalent in Pakistan. The study was aimed to isolate Mccp local strain for the development of diagnostics and vaccines.

Methods

Samples were collected during November 2017–December 2018 at Northern areas of Pakistan from 10 goat flocks each in Gilgit-Baltistan, Chitral, Swat, Buner, and Hazara. 900 samples were collected; nasal swabs (n = 400), tracheal swabs (n = 150) from naturally infected goats showing clinical signs of CCPP, and lungs tissue (n = 200), pleural fluid (n = 150) from goats at necropsy.

Results

The clinical signs recorded were mucopurulent nasal discharges, cough, abdominal respiration and hyperthermia. The post-mortem revealed, pulmonary consolidation, fibrinous pleuropneumonia, and accumulation pleural fluid. The fried egg like growth was observed on agar in 16 (4%), 11 (7.3%), 38 (19%), and 24 (16%) nasal swab, tracheal swabs, lungs and pleural fluid samples, respectively. PCR targeting 16S rRNA gene revealed isolates, belongs to Mycoplasma mycoides cluster, in 72 (8%) samples. Forty one (4.5%) isolates were Mccp by specie specific PCR generating an amplicon of 316 bp.

Conclusions

We successfully isolated local strain of Mccp for the first time in Pakistan. This Mccp strain could be further utilized for the development of diagnostics and control measures against Mccp infection in goats.

中文翻译：

---

山羊支原体亚种的首次分离和分子表征。capripneumoniae巴基斯坦株：传染性山羊胸膜肺炎的病原体

目的

山羊支原体亚种。capripneumoniae（MCCP）导致在被称为传染性胸膜肺炎山羊（CCPP）山羊严重的，通常是致命的疾病。CCPP 被 OIE 列为应报告的动物疾病，在高发病率和死亡率方面造成经济损失。迄今为止，关于巴基斯坦流行的独特McCp菌株的分子特征的信息非常有限。该研究旨在分离 McCp 本地菌株以开发诊断方法和疫苗。

方法

2017 年 11 月至 2018 年 12 月期间，在巴基斯坦北部地区从吉尔吉特-巴尔蒂斯坦、吉特拉尔、斯瓦特、布内尔和哈扎拉的 10 个山羊群中收集了样本。收集了 900 个样本；鼻拭子 (n = 400)、自然感染山羊的气管拭子 (n = 150) 显示 CCPP 的临床症状，以及肺组织 (n = 200)、尸检时山羊的胸水 (n = 150)。

结果

记录的临床症状是粘液脓性鼻涕、咳嗽、腹部呼吸和体温过高。尸检显示，肺实变、纤维蛋白性胸膜肺炎和胸水积聚。在琼脂上分别观察到 16 (4%)、11 (7.3%)、38 (19%) 和 24 (16%) 个鼻拭子、气管拭子、肺和胸水样本中的煎蛋样生长。PCR 靶向 16S rRNA 基因显示分离株，属于支原体菌群，在 72 (8%) 个样本中。四十一个 (4.5%) 分离株是通过物种特异性 PCR 产生的Mccp，产生 316 bp 的扩增子。

结论

我们首次在巴基斯坦成功分离了本地的Mccp菌株。该McCp菌株可进一步用于开发针对山羊中 McCp 感染的诊断和控制措施。