Bacillus coagulans T242 has high lactase-producing activity and the lactase produced is thermally stable. However, the probiotic and other functional properties of the bacterium had not been studied and the bacterium needed further investigation for potential use in food products. This study aimed to evaluate the probiotic and functional characteristics of B. coagulans T242 in vitro. B. coagulans T242 has a high resistance to simulated gastric juice (pH 3.0) and bile salt (0.3%), indicating that it may have a good tolerance of gastrointestinal conditions. The adhesion of B. coagulans T242 to HT-29 cells was calculated as 58%. Additionally, B. coagulans T242 reduced the adhesion of Escherichia coli to HT-29 cells by up to 62%. B. coagulans T242 is sensitive to amoxicillin, amikacin, tetracycline, chloramphenicol, and rifampin, and is moderately sensitive to ciprofloxacin and erythromycin. These characteristics showed that B. coagulans T242 is a potential probiotic bacterium. B. coagulans T242 showed good cholesterol assimilation potential (19.9%) in vitro. In addition, B. coagulans T242 showed a high capacity for scavenging 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radicals (35.0%), hydroxyl radicals (39.0%), and superoxide anion radicals (14.8%), and good reducing power (58.5 μmol/L ascorbic acid equivalent). It was speculated that B. coagulans T242 might have the potential to exert antioxidant activity in vitro. These preliminary results suggested that B. coagulans T242 has the potential to be used as a probiotic.

凝结芽孢杆菌T242具有高的乳糖酶产生活性，并且产生的乳糖酶是热稳定的。但是，尚未研究细菌的益生菌和其他功能特性，需要对该细菌进行进一步研究以潜在地用于食品中。这项研究的目的是评估的益生菌和功能特性，凝结芽孢杆菌T242体外。凝结芽孢杆菌T242对模拟胃液（pH 3.0）和胆汁盐（0.3％）具有很高的抵抗力，表明它可能对胃肠道疾病具有良好的耐受性。凝结芽孢杆菌T242对HT-29细胞的粘附计算为58％。此外，凝结芽孢杆菌T242减少了大肠杆菌对HT-29细胞的作用高达62％。凝结芽孢杆菌T242对阿莫西林，阿米卡星，四环素，氯霉素和利福平敏感，对环丙沙星和红霉素中等敏感。这些特征表明，凝结芽孢杆菌T242是潜在的益生菌。凝结芽孢杆菌T242在体外显示出良好的胆固醇同化潜力（19.9％）。此外，凝结芽孢杆菌T242具有较高的清除1,1-二苯基-2-吡啶并肼基（DPPH）自由基（35.0％），羟基自由基（39.0％）和超氧阴离子自由基（14.8％）的能力，并且良好。还原能力（58.5μmol/ L抗坏血酸当量）。据推测凝结芽孢杆菌T242可能具有在体外发挥抗氧化活性的潜力。这些初步结果表明凝结芽孢杆菌T242具有用作益生菌的潜力。