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Antioxidant Activity of Crude Extracts and Essential Oils from Flower Buds and Leaves of Cistus creticus and Cistus salviifolius
Arabian Journal of Chemistry ( IF 6 ) Pub Date : 2020-07-01 , DOI: 10.1016/j.arabjc.2020.05.043
Sultan T. Abu-Orabi , Mahmoud A. Al-Qudah , Noura R. Saleh , Tareq T. Bataineh , Safwan M. Obeidat , Mohammed S. Al-Sheraideh , Hala I. Al-Jaber , Hasan I. Tashtoush , Jamil N. Lahham

Abstract Volatile oils from flowers and leaves of C. creticus L. and C. salviifolius L. were extracted by two extraction methods; namely, hydrodistillation and solid-phase micro-extraction (SPME). The chemical composition of essential oils was analyzed by GC and GC–MS. The volatile extracted from leaves and flowers of C. criticus using SPME was dominated by monoterpenes and sesquiterpenes hydrocarbon with α-pinene, camphene and α-cubebene as major components. In hydrodistillation, the oil extracted from leaves was dominated by oxygenated diterpenes and diterpenes hydrocarbon with manoyl oxide and sclarene as major components, whereas, the oil extracted from flowers was dominated by oxygenated diterpenes and diterpenes hydrocarbon with manoyl oxide and abietatriene as major components. The volatile from flowers and leaves of C. salviifolius obtained by SPME were dominated by monoterpenes and sesquiterpenes with δ-3-carene, α-pinene, β-pinene, and E-caryophyllene as major constituents. On the other hand, the oils from flowers and leaves of C. salviifolius obtained by hydrodistillation were dominated by oxygenated diterpenes, diterpenes hydrocarbon and esters with dehydro abietol, abietol, manoyl oxide and methyl octadecenoate as major components. In the leaves, the major components of the oil were manoyl oxide, E-ethyl cinnamate, and Z-ethyl cinnamate. These oils showed weak antioxidant activity when compared to the positive controls α-tocopherol, ascorbic acid, and EDTA, while the crude extracts aq. MeOH, butanol, and water showed good antioxidant activity. Discriminating between the studied plants based on the extraction method was also possible upon applying Principle component analysis (PCA) to the obtained GC–MS data.

中文翻译:

岩蔷薇和岩蔷薇花芽和叶子的粗提取物和精油的抗氧化活性

摘要 采用两种提取方法提取了C. creticus L.和C. salviifolius L.花和叶中的挥发油;即水蒸馏和固相微萃取 (SPME)。通过GC和GC-MS分析精油的化学成分。用SPME从C.critus叶和花中提取的挥发物以单萜和倍半萜烃为主,以α-蒎烯、莰烯和α-立方烯为主要成分。在水蒸馏中,从叶子中提取的油以氧化二萜和二萜烃为主,以甘露醇和紫杉烯为主要成分,而从花中提取的油以氧化二萜和二萜烃为主,以甘露醇和松香三烯为主要成分。花叶挥发物 C. SPME 获得的丹参以单萜和倍半萜为主,主要成分为 δ-3-蒎烯、α-蒎烯、β-蒎烯和 E-石竹烯。另一方面,通过水蒸馏获得的丹参花和叶油以含氧二萜、二萜烃和酯为主,以脱氢松香醇、松香醇、氧化甘露醇和十八碳烯酸甲酯为主要成分。在叶子中,油的主要成分是氧化甘露酸、E-肉桂酸乙酯和肉桂酸Z-乙酯。与阳性对照 α-生育酚、抗坏血酸和 EDTA 相比,这些油显示出较弱的抗氧化活性,而粗提物水溶液。MeOH、丁醇和水显示出良好的抗氧化活性。
更新日期:2020-07-01
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