Abstract

Proteolytic activity of 8 strains of micromycetes (belonging to different ecological and trophic groups: phyto-, nemato-, myco-, and entomopathogens as well as human pathogens) toward the hemostatic system proteins has been assayed by using the chromogenic peptide substrates. The investigated micromycetes are shown to secrete proteases with a broad substrate specificity. However, there are producers among them exhibiting a pronounced plasmin-like and urokinase activity (Arthrobotrys longa 1, Sarocladium strictum 203) as well as a tPA (tissue plasminogen activator)-like activity (Purpureocillium lilacinum k1). It is found that the most active producer of proteases with fibrinolytic and plasminogen activator activity is S. strictum 203. Plasminogen activator activity of proteinases produced by S. strictum 203 is approximately 50% of the fibrinolytic one, which makes this strain a promising producer of fibrinolytic enzymes.

中文翻译：

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蛋白酶的微真菌分泌，其活性类似于止血系统蛋白的活性

摘要

已使用生色肽底物测定了8种微霉菌菌株（属于不同的生态和营养类：植物病原，线虫病，霉菌病和昆虫病原体以及人类病原体）的蛋白水解活性。已显示所研究的微真菌分泌具有广泛底物特异性的蛋白酶。但是，其中一些生产商表现出明显的纤溶酶样和尿激酶活性（Arthrobotrys longa 1，Sarocladium strictum 203）以及tPA（组织纤溶酶原激活物）激活（Purpureocillium lilacinum k1）。发现具有纤溶酶和纤溶酶原激活物活性的最活跃的蛋白酶生产者是严格链球菌。203.严格链球菌203产生的蛋白酶的纤溶酶原激活剂活性约为纤维蛋白溶解酶的50％，这使该菌株成为纤维蛋白溶解酶的有前途的生产者。